慢速冷冻胚胎TET周期当天与解冻后培养1d移植结果分析  

Outcome of Frozen-thawed Embryo Transfer between Different Culture Time In Vitro in Natural Cycles and Artificial Cycles

作　　者：李瑞岐[1] 王文军[1] 麦美琪[1] 欧阳能勇[1] 马芸[1] 陈静华[1] 张宁锋[1] 

机构地区：[1]中山大学孙逸仙纪念医院妇产科生殖医学中心,广东广州510120

出　　处：《中山大学学报（医学科学版）》2011年第6期824-827,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2010B031600043);广东省人口计生委科研项目(2009231)

摘　　要：【目的】探讨第3天(D3)慢速冷冻胚胎解冻后当天行胚胎移植(ET)和体外培养1 d后ET在自然周期和人工周期中对临床结局的影响。【方法】回顾性分析本中心2009年1月至2010年12月期间的解冻胚胎数≥1,且≤3的552个卵裂期冷冻胚胎TET周期的临床结果,根据解冻后体外培养时间分为A组(当天ET)和B组(培养1 d后ET),这两个组再分为自然周期组和人工周期组。【结果】D3冷冻胚胎在自然周期和人工周期,A、B两组女方年龄、平均移植胚胎数、胚胎评分以及妊娠率均没有统计学意义(P>0.05),但是,在自然周期和人工周期中B组种植率较A组均有统计学意义(17.9 vs 12.3,P<0.05;17.3%vs 8.7%,P<0.05)。【结论】体外培养时间对D2冷冻胚胎临床结局没有影响,而D3冷冻胚胎TET时,解冻后培养1 d再进行ET有利于胚胎种植。[Objective] To assess the outcome of slow-frozen/thawed embryo transfer （TET） between different culture time of the embryos frozen at day 3 （D3） in vitro in natural and artificial cycles. [ Methods ] A database was analyzed retrospectively concerning TET cycles covered 552 cycles, in which the number of thawed embryos 〉/1 and ~〈3 from February 2009 to December 2010. According to the different culture time after embryo thawing, they were divided into group A（ET at the thawing day） and group B （ET at the next day offer embryo thawed）. And these two groups were further divided into nature cycle and artificial cycle. [ Results] Although there were not significantly different between the couples age, the number of embryos transferred and the embryos scale （P 〉 0.05 ）.The implantation rates of D3 frozen embryos in group B were significantly higher than those in group A in both of the nature cycles and the artificial cycles （17.9 vs 12.3 ,P 〈 0.05; 17.3% vs 8.7%,P 〈 0.05）, respectively.[Conclusion] The effect of extending incubation time of thawed embryos does not significantly effect the outcome of D2 freezing embryos. But in the TET cycles of D3 frozen embryos, ET at the next day of embryo thawed will be better for the embryo implantation.

关 键 词：冻融胚胎移植 移植时间 妊娠率 种植率 自然周期 人工周期 

分 类 号：R711.6[医药卫生—妇产科学]