双抗体夹心二步法检测甲胎蛋白后带现象  

The Postzone Phenomenon of AFP Detection in Blood by Double Antibody Sandwich Method

作　　者：吴巧萍 穆银玉 陈兵华 谢晶晶[2] 

机构地区：[1]宁波市李惠利医院检验科,315041 [2]绍兴文理学院

出　　处：《医学研究杂志》2011年第12期126-127,共2页Journal of Medical Research

摘　　要：目的探讨用双抗体夹心二步法检测甲胎蛋白(AFP)出现"后带现象"而造成检测值偏低的发生原因及解决方法。方法把2份临床诊断为原发性肝癌但AFP测定结果较低的标本按原液至1∶400手工倍比稀释后,在ABBOTT公司的Architecti2000 System自动免疫化学发光分析仪上采用化学发光微粒子免疫分析法(CMIA)测定,然后对结果进行分析。结果测定结果分别为194μg/L和284μg/L的2份样本原液,经手工倍比稀释后上机检测,前者检测结果约为10万μg/L,后者检测结果约为8万μg/L。结论双抗体夹心二步法(CMIA,雅培)检测高浓度的AFP也存在"后带现象",须经稀释后才能得到可靠结果。Objective To investigate the reasons of the postzone phenomenon in detection of AFP in blood by double antibody sandwich method.Methods Two specimens of lower results which were diagnosed as primary hepatic carcinoma（PHC） in clinical practice were multiple proportion diluted to 1∶ 400,then were inspected by Architect i2000 System of ABBOTT company applying CMIA.Finally the measured values were analyzed.Results The two specimens whose original measured value 194μg/L and 284μg/L were detected again after handle multiple proportion dilution.The final results were approximately 100000μg/L and 80000μg/L,respectively.Conclusion Postzone phenomenon also exists when high concentration AFP specimen is measured by double antibody sandwich method（CMIA,Architect）.Pathologists should dilute the serum in multiple proportions in order to get correct results.

关 键 词：化学发光微粒子免疫分析法(CMIA) 双抗体夹心二步法 AFP 后带现象 

分 类 号：R446.62[医药卫生—诊断学]