罗格列酮对实验性1型糖尿病大鼠胰岛β细胞凋亡的影响  

Effect of rosiglitazone on the apoptosis of beta cells in rats with experimental type 1 diabetic mellitus

作　　者：高会敏[1] 蒙碧辉[2] 韦静彬[3] 陈洪流[4] 林媛媛[5] 

机构地区：[1]河南省周口市中心医院内分泌科,周口466000 [2]广西医科大学第一附属医院内分泌科,南宁530021 [3]广西柳州市人民医院传染科,柳州545006 [4]广西医科大学第一附属医院急诊科,南宁530021 [5]广西南宁市第一人民医院内分泌科,南宁530021

出　　处：《重庆医科大学学报》2011年第11期1304-1306,共3页Journal of Chongqing Medical University

基　　金：广西科学基金资助项目(编号:桂科自0640130)

摘　　要：目的:观察罗格列酮(Rosiglitazone natrium,RSG)对实验性1型糖尿病大鼠胰岛β细胞凋亡的影响。方法:6周龄SD大鼠随机分成3组,正常对照(Control,Con)组24只,糖尿病(Diabetic mellitus,DM)组24只,罗格列酮干预(Rosiglitazone,RSG)组24只。以链脲佐菌素(Streptozotocin,STZ)溶液单次腹腔注射,72 h后测空腹血糖(Fasting blood glucose,FBG)≥16.7 mmol/L为造模成功。应用罗格列酮后5个时间段(2、5、7、10、13周),分别用TUNEL(Terminal deoxynucleotidyl transferase dUTP nickend labeling)方法检测胰岛β细胞的凋亡,用免疫组化法检测胰岛中胰岛素(Insulin,Ins)及Fasl的表达。结果:与DM组相比,RSG组胰岛表达胰岛素水平提高,β细胞相对凋亡指数及Fasl表达水平均下降(P均<0.05)。结论:罗格列酮能抑制或延缓β细胞凋亡,其机制可能与抑制Fas/Fasl细胞凋亡通路有关。Objective：To explore the effect of rosiglitazone on the apoptosis of pancreatic beta cells in rats with experimental type 1 diabetic mellitus.Methods：Sprague-Dawley rats were randomly divided into control group（Con,n=24） and diabetic control group（DM,n=24） and rosiglitazone group（RSG,n=24）.Diabetic rats were induced by streptozotocin（STZ） injected intraperitoneally.Rats with fasting blood glucose（FBG）≥16.7 mmol/L were served as diabetic model.Two-,five-seven-,ten-,and thirteen weeks after RSG treatment,the apoptosis of beta cell was evaluated by Terminal deoxynucleotidyl transferase dUTP nick end labeling（TUNEL） assay and the levels of expression for insulin and Fasl were performed by immunohistochemistry.Results：Two weeks after RSG treatment,the levels of expression for insulin in the pancreas islet were higher（P0.05） and the relative index of apoptosis of beta cells was lower（P0.05） coupling a downgraded expression of Fasl（P0.05） in RSG than that in DM.Conclusion：Rosiglitazone can inhibit or down regulating the apoptosis of beta cells to some extent in experimental type 1 diabetic rats,and one of the mechanisms was probably through inhibiting the apoptosis pathway of Fas/Fasl.

关 键 词：罗格列酮 细胞凋亡 糖尿病 FAS/FASL 胰岛Β细胞 

分 类 号：R587.1[医药卫生—内分泌]