HCG刺激对未成熟卵母细胞体外成熟培养的影响  

Effect of HCG-stimulation on in-vitro maturation of immature mouse oocytes

作　　者：王炼炼[1] 丘彦[1] 幸贵邦[1] 

机构地区：[1]重庆医科大学附属第一医院辅助生殖中心,重庆400016

出　　处：《重庆医科大学学报》2011年第11期1322-1324,共3页Journal of Chongqing Medical University

摘　　要：目的:探讨获取未成熟卵母细胞之前使用人绒毛膜促性腺激素(Human chorionic gonadotropin,HCG)能否改善未成熟卵母细胞在体外培养条件下成熟的时间、体外受精率、2细胞、4细胞发生率。方法:将HCG刺激后或未刺激所获得的小鼠生殖泡(Germinal reside,GV)期卵丘-卵母细胞复合物在培养液中进行体外培养,观察未成熟卵母细胞减数分裂的恢复、核成熟、受精率以及胚胎的发育。结果:体外培养后16 h G-PR组生殖泡破裂(Germinal vesicle break down,GVBD)发生率显著高于G-UPR组(P<0.01)。体外培养后20 h G-PR组GVBD发生率明显高于G-UPR组(P<0.05)。体外培养后24 h G-PR组PBI发生率显著高于G-UPR组(P<0.01)。G-PR组受精率明显高于G-UPR组(P<0.05)。结论:HCG加快了卵母细胞的体外成熟进程,同时也增加了卵母细胞的体外受精率。Objective：To investigate the in-vitro maturation time,in vitro fertilization,2 cell and 4 cell rate of the immature oocytes after the use of HCG（human chorionic gonadotropin）.Methods：The germinal vesicle（GV） immature oocytes were acquired from the ovaries of the HCG（human chorionic gonadotropin）-stimulated and non-HCG-stimulated rats.Immature oocytes were cultured in culture media.Observations were made for the status of germinal vesicle break-down（GVBD） and the extrusion of the first polar body（PB1）.The achieved MⅡoocytes were then inseminated in-vitro;and the pronuclei（PN） and the pre-implantation development were observed.Results：Observations showed that after being cultured via in-vitro maturation in culture media for 16 hours,the GVBD in the GV cumulus-oocyte complexes（COC） occurring in the HCG-stimulated group had better outcomes than in non-HCG-stimulated group（P0.01）.After being cultured for 20 hours the GVBD occurring in the HCG-stimulated group had better outcomes than in non-HCG-stimulated group（P0.01）.After being cultured for 24 hours,the elimination ratio of PB1 in the HCG-stimulated group was higher than that of the non-HCG-stimulated group（P0.01）.The fertilization rate of the oocytes in the HCG-stimulated group was higher than that in non-HCG-stimulated group（P0.05）.Conclusion：Stimulation with HCG can speed up the maturation of the oocytes,and enhance the fertilization rate.

关 键 词：绒促性腺激素 卵母细胞 体外成熟 

分 类 号：R714.13[医药卫生—妇产科学]