宁夏回、汉族亚甲基四氢叶酸还原酶基因多态性的分布特点及其与原发性高血压的关系  

A study of gene ploymorphism of N5,10-methylenetetrahydrofolate reductase (MTHFR) between Hui and Han nationalities in Ningxia and the relationship with essential hypertension

作　　者：马萍[1] 覃数[1] 刘海燕[2] 徐清斌[2] 

机构地区：[1]重庆医科大学附属第一医院心内科,重庆400016 [2]宁夏医科大学总医院心脏中心,银川750004

出　　处：《重庆医科大学学报》2011年第11期1364-1367,共4页Journal of Chongqing Medical University

基　　金：宁夏回族自治区自然科学基金资助项目(编号:NZ10134)

摘　　要：目的:研究N5,10-亚甲基四氢叶酸还原酶基因(Methylenetetrahydrofolate reductase,MTHFR)C677T多态性在宁夏回、汉族高血压人群中的分布差异及其与原发性高血压(Essential hypertension,EH)之间的关系。方法:收集回、汉族EH患者146例和155例以及回、汉族健康体检正常血压者(Normal tensive,NT)112例和140例,采用聚合酶链反应-限制性片段长度(Poly-merase chain reaction-restriction fragment length polymorphism,PCR-RFLP)多态性技术分析检测MTHFR基因多态性。结果:回、汉族EH组间比较基因型频率及等位基因频率均无统计学差异(P>0.05)。回、汉族NT组间比较基因型频率和等位基因频率亦无统计学差异(P>0.05)。回族EH组TT基因型频率及T等位基因频率均高于回族NT组(P<0.05)。汉族EH组变异型TT+CT及T等位基因频率均高于汉族NT组(P<0.05)。二项逐步Logistic回归结果显示,TT基因型为EH的独立危险因素(OR=2.34,95%CI为1.363～4.016,P=0.002)。结论:MTHFR基因C677T多态性在回、汉族人群之间无种族差异;该位点多态性与宁夏地区回、汉族人群EH发病具有相关性,T等位基因有可能是EH的遗传易感基因。Objective：To comparatively study distributional characteristics of gene ploymorphism of N5,10-methylenetetrahydrofolate reductase（MTHFR） between Hui and Han nationalities in Ningxia and the relationship between the polymorphism and essential hypertension. Methods ：The study was performed in 146 Hui patients and 155 Han patients with essential hypertensive （EH） and was performed in 112 Hui peoples and 140 Han peoples with normal tensive（NT）. MTHFR gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）. Results：The frequencies of Tr genotype and T allele in Hui patients with hypertension were of no difference with those in Han patients（P〉0.05）. There were also no significant differences between Hui NT group and Han NT group（P〉0.05）. The frequencies of 677TF genotype and T allele in Hui patients with EH were higher than those of Hui NT group（P〈0.05）. The frequencies of CT＋TY genotype and T allele in Han patients with EH were both higher than those of control（P〈0.05）. Binary stepwise logistic regression analysis showed that TT genetype（OR=2.34,95%CI 1.363-4.016, P= 0.002） was independent risk factor for essential hypertension. Conclusion：There does not exist racial difference between Hui and Han nationalities with MTHFR C677T gene polymorphism. The polymorphism has a correlation with EH between Hui and Han nation- alities in Ningxia. T allele maybe a susceptible gene for EH in Ningxia people.

关 键 词：N5 10-亚甲基四氢叶酸还原酶 多态性 宁夏 民族 高血压 

分 类 号：R544.1[医药卫生—心血管疾病]