陆地棉超矮杆突变体基因的初步定位  

Gene mapping of an ultra-dwarf mutant in upland cotton

作　　者：景超[1] 马晓杰[2] 狄佳春[2] 陈旭升[2] 

机构地区：[1]南京农业大学农学院,南京210095 [2]江苏省农业科学院,农业部长江下游棉花与油菜重点实验室,南京210014

出　　处：《遗传》2011年第12期1393-1397,共5页Hereditas（Beijing)

基　　金：转基因生物新品种培育科技重大专项子课题(编号:2008ZX08005-001);江苏省农业科技自主创新资金项目(编号:CX 09-115)资助

摘　　要：陆地棉超矮杆是一种新发现的突变体材料。前期对其遗传规律的研究表明,超矮杆突变性状是受一对完全隐性基因du控制的质量性状。文章以"超矮1号"和"新陆早16"配置的F2为作图群体,通过对双亲以及近等基因池的筛选,在1 350对SSR引物中共获得70对多态性引物。而后检测F2作图群体每个单株的基因型,显示有36个标记可以连锁,分布在8个连锁群上,其中超矮杆突变性状位于连锁群LG01。与目标基因du连锁的分子标记有7个:NAU2679、NAU2749、NAU905、NAU2838、NAU5373、NAU2238和NAU4946,它们皆为共显性标记。依据现有的棉花遗传图谱,标记NAU4946、NAU2238、NAU905、NAU5373和NAU2679位于第6染色体上,而目标基因du位于NAU2238和NAU4946之间,遗传距离分别为3.3 cM和1.4 cM,由此推定du在第6条染色体上。A ultra-dwarf mutant was newly found in upland cotton（Gossypium hirsutum L.）.This mutant was controlled by single recessive gene du.Seventy pairs of polymorphic primers were selected from 1350 primers,which covered all the identified chromosomes by screening in parents and near-isogenic lines.Gene du was mapped using an F2 population derived from intraspecific crosses between ＂Ultra-dwarf 1＂ and ＂Xinluzao 16＂.Thirty-six primers were distributed in eight linkage groups,and du was linked to LG01.Seven co-dominant markers linked to du were NAU2679,NAU2749,NAU905,NAU2838,NAU5373,NAU2238,and NAU4946.Based on the known genetic map of tetraploid cotton,the markers NAU4946,NAU2238,NAU905,NAU5373,and NAU2679 were located on chromosome 6,and the target gene du was located between NAU2238 and NAU4946 with the genetic distances of 3.3 cM and 1.4 cM,respectively.Hence,gene du was located on chromosome 6.

关 键 词：陆地棉 超矮杆突变体 基因定位 SSR 

分 类 号：S562[农业科学—作物学]