γ射线诱导小鼠胸腺淋巴细胞中Arp2、Arp3和cofilin基因表达的变化  

Expression changes of Arp2,Arp3 and cofilin genes of lymphocytic microfilament in thymus gland induced by γ-rays in BALB/c mice

作　　者：齐雪松[1] 王春燕[1] 郝述霞[1] 苟巧[1] 佟鹏[1] 苏旭[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《癌变．畸变．突变》2011年第6期421-424,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：公益性卫生行业科研专项项目(200802018)

摘　　要：目的:观察不同剂量的γ射线照射小鼠后,胸腺淋巴细胞中Arp2、Arp3和cofilin基因表达的变化。方法:BALB/c小鼠40只,随机分成4个照射组和1个非照射组,每组8只。照射组行全身γ射线照射,照射剂量分别为0.02、0.1、1和6 Gy。于照后3 h颈椎脱臼处死小鼠,取出胸腺,用PBS冲出胸腺淋巴细胞。分别采用荧光定量PCR技术和Western-blot检测各组小鼠胸腺淋巴细胞中Arp2、Arp3、cofilin mRNA和蛋白的表达。结果:与未照射组比较,各照射组小鼠胸腺淋巴细胞Arp2 mRNA表达的变化无统计学意义(P>0.05),1 Gy照射组Arp2蛋白表达上调(H=2.33,P<0.05);1 Gy照射组Arp3 mRNA表达下调(t=-12.77,P<0.01),6Gy照射组Arp3 mRNA表达上调(t=3.39,P<0.05),各照射组Arp3蛋白的表达无明显变化;0.02、0.1和1 Gy照射组cofilin mRNA的表达上调(t=6.38～9.41,P<0.01),各照射组cofilin蛋白的表达变化无统计学意义(P>0.05)。结论:不同剂量的γ射线可诱导BALB/c小鼠胸腺淋巴细胞Arp2、Arp3和cofilin基因表达发生变化,其变化特点与照射剂量大小有关,该结果为研究辐射致胸腺淋巴细胞微丝骨架改变的机制提供了实验依据。OBJECTIVE：To study the expression changes of Arp2,Arp3 and cofilin genes of lymphocytic microfilament in thymus gland induced hy different doses ofγ-rays in BALB/c mice.METHODS：40 BALB/c mice were divided into five groups.Four groups were separately exposed toγ-rays with 0.02,0.1,1 and 6 Gy in whole body. One group was control.Every group had 8 mice.After 3 hours induced byγ-rays,the mice were given cervical dislocation,chests and thymuses were collected.Thymus cells were flowed out by PBS immited.mRNA and proteins of Arp2,Arp3 and cofilin genes were extracted from thymus cells.Their mRNA expressions proteins were detected by fluorescent quantitation PCR.The Arp2,Arp3 and cofilin proteins were measured by western-blot.RESULTS： Compared with control,mRNA expressions of Arp2 gene induced byγ-rays with different doses were not statistically significant,but Arp2 protein expressions induced byγ-rays with 1Gy were upward（H=2.33,P〈0.05）;mRNA expressions of Arp3 gene induced byγ-rays with 1 Gy were downward（t=-12.77,,P〈0.01）,mRNA expression of Arp3 gene induced by 6 Gyγ-rays were upward（t=3.39,P〈0.05）,differences of Arp3 protein expressions induced byγ- rays with different doses were not statistically significant;mRNA expressions of cofilin gene of groups induced seperately byγ-rays with 0.02,0.1 and 1 Gy were upward（t=6.38-9.41,P〈0.01）,while differences of cofilin protein expressions induced byγ-rays with different doses were not statistically significant.CONCLUSION：Afterγ-rays induced with different doses,the expressions changes of Arp2,Arp3 and cofilin genes took place in thymus gland of B ALB/c mice. Characteristics of the their changes were associated with doses ofγ-rays.These results provided experimental evidences for the mechanism of changes of lymphocyteic microfilament meshwork induced byγ-rays.

关 键 词：Γ射线 淋巴细胞 胸腺 Arp2 Arp3 COFILIN 

分 类 号：Q506[生物学—生物化学]