γ射线辐照对BEP2D细胞蛋白质组的影响  

γ-rays irradiation effects on proteomics of lung cell line BEP2D

作　　者：薛振伟[1] 周小林[1] 崔梅萍[1] 

机构地区：[1]中国辐射防护研究院,山西太原030006

出　　处：《癌变．畸变．突变》2011年第6期425-428,433,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金："十一五"国防基础科研项目(A0120060527-06)

摘　　要：目的:利用蛋白质组学方法,研究与人永生化支气管上皮细胞(BEP2D细胞)辐照相关的差异蛋白质,寻找辐射致肺癌早期诊断和治疗的新指标。方法:分别培养并收集了0、1.5和3.0 Gyγ射线辐照24 h后的BEP2D细胞样品,用双向凝胶电泳技术分离照射组与对照组细胞样品中的总蛋白质,应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)得到相应的肽质量指纹图,并最终鉴定差异蛋白质。结果:质谱鉴定获得10张肽质量指纹图,经数据库搜索后8个蛋白点被初步鉴定(2个蛋白点无结果),其中1个蛋白点重复。用Mascot软件查询NCBInr数据库初步鉴定出7种差异表达的蛋白质,即亲环素A、肌球蛋白轻链2、细胞角蛋白9、α-烯醇酶、磷酸丙糖异构酶1、Makorin环指蛋白1、c-myc启动子结合蛋白1。结论:所鉴定的7种蛋白与辐射诱导肺癌的发生、发展过程密切相关,为寻找辐射致肺癌早期诊断和治疗的新靶点提供了理论依据。OBJECTIVE：To identify the specific tumor markers of lung cancer induced by irradiation for diagnose and therapy by proteomics.METHODS：Samples of BEP2D cells irradiated with 0,1.5 and 3.0 Gyγ-rays for 24 h were cultivated and collected,and total protein was separated with the two-dimensional gel electrophoresis.The peptide mass fingerprints were obtained using matrix-assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS）for differentially expressed proteins.RESULTS：10 peptide mass fingerprinting were revealed in the mass spectra identification,8 of them approved in the database（2 null）,and 1 duplicated.There were 7 points identified in the initial tests by Mascot software in the NCBInr databates,such as cyclophilin A,myosin light chain 2,cytokeratin 9,α-enolase,triosephosphate isomerase 1,makorin ring finger protein 1,c-myc promoter-binding protein 1.CONCLUSION：The 7 proteins probably were the special proteins related to lung cancer induced by irradiation,which might be used for further study of the functions of the proteins.

关 键 词：蛋白质组 BEP2D细胞 γ射线 亲环素A 肌球蛋白轻链2 

分 类 号：R818.03[医药卫生—放射医学]