单细胞凝胶电泳技术用于估算大剂量电离辐射的初步探索  

High dose radiation-induced DNA damage using single cell gel electrophoresis

作　　者：段志凯[1] 时爱丽[2] 刘建功[1] 王斌生[2] 郭万龙[1] 张淑贤[1] 

机构地区：[1]中国辐射防护研究院,山西太原030006 [2]山西省荣军医院,山西太原030006

出　　处：《癌变．畸变．突变》2011年第6期442-445,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：山西省自然科技基金项目(2009011049-4)

摘　　要：目的:初步探索碱性单细胞凝胶电泳(single cell gel electrophoresis,SCGE)方法用于大剂量电离辐射生物剂量估算的可行性。方法:用不同剂量水平(0～16Gy)的^(60)Coγ射线照射正常人离体外周血,分别于照射后即刻和1 h后进行SCGE检测,用CASP软件分析血细胞"彗星"尾矩(tailmoment,TM),建立剂量-效应曲线。结果:外周血细胞"彗星"尾矩随着照射剂量的增加而增加。以"彗星"尾矩为指标,使用统计软件Origin 7.5进行拟合,受照即刻在0～16 Gy剂量范围内彗星TM值与受照剂量D之间的剂量-效应曲线为曲线模式,曲线方程为Y=0.836 7+0.530 9D+0.079 9D^2(R^2=0.996 2,P<0.05),而0～8Gy剂量范围内剂量-效应曲线为线性模式,直线方程为Y=0.400 3+1.129 1D(R^2=0.996 3,P<0.05)。受照1h后0～16Gy剂量范围内,彗星TM值与受照剂量D之间的关系曲线为Y=0.461 6+0.6560D+0.062 1D^2(R^2=0.9984,P<0.05),0～8 Gy剂量范围内的关系曲线为Y=0.107 1+1.128 3D(R^2=0.999 5,P<0.05)。结论:用碱性SCGE分析淋巴细胞"彗星"尾矩有望成为一种估算大剂量电离辐射的生物剂量计。OBJECTIVE：To explore the feasibility of single cell gel electrophoresis（SCGE） in estimation of radiation biological dose.METHODS：Normal peripheral blood samples from two healthy males were exposed to different doses 60Coγrays,ranged from 0 to 16 Gy.Immediately and 1 h later,tail moment（TM） of the peripheral blood cells were analyzed with casp software in order to establish dose-effect relationship.RESULTS：The TMs immediately after radiation of peripheral blood cells increased with the irradiation doses,and its relationship can be fitted as：Y=0.836 7＋0.530 9D＋0.0199D^2（R2=0.9962,P〈0.05） from 0 to 16 Gy,and Y=0.4003＋1.1291D（R2=0.9963,P〈0.05） from 0 to 8 Gy.The TMs of peripheral blood cells 1 h after radiation increased with the irradiation doses,and its relationship can be fitted as：Y=0.4616＋0.6560D＋0.062 1 D2（R2=0.9984,P〈0.05） from 0 to 16 Gy,and Y=0.1071＋1.1283D（R2=0.999 5,P〈0.05） from 0 to 8 Gy.CONCLUSION：The TM of peripheral blood cells analyzed with SCGE is a promising radiation biological dosimeter at high doses.

关 键 词：单细胞凝胶电泳 电离辐射 外周血 剂量-效应关系 

分 类 号：TL818[核科学技术—核技术及应用]