外周血网织红细胞和T淋巴细胞亚群用于辐射损伤快速剂量估算的可行性研究  

A feasibility study on rapid radiation damage estimation using peripheral blood reticulocytes and T cell subsets

作　　者：王雷[1,2] 王治东[2] 胡海亮[1,2] 张学清[2] 陈茂胜[2] 薛绍礼[1] 陈英[2] 

机构地区：[1]安徽医科大学医学生物工程教研室,安徽合肥230032 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《癌变．畸变．突变》2011年第6期452-455,459,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：军队医药卫生科研基金项目(08G143)

摘　　要：目的:通过观察受不同剂量照射小鼠的外周血网织红细胞的百分率变化以及辅助T淋巴细胞(helperT cells,Th)与抑制T淋巴细胞(suppressor T cells,Ts)的比值(r_(Th/Ts))和照射剂量的关系,探索其用于辐射损伤生物剂量估算的可行性。方法:C57BL/6小鼠,7～8周龄,^(60)Coγ射线全身照射0～7Gy,分别收集辐照前和辐射后不同时间点的静脉血,流式细胞术(FCM)检测外周血中网织红细胞百分率和r_(Th/Ts)情况,分析时间-效应关系并拟合剂量-效应曲线。结果:照射剂量在0～7 Gy范围内,小鼠的外周血网织红细胞百分率均随时间而降低,并于照射后3 d降到最低,且剂量越大恢复越晚;照射后72 h内小鼠的外周血r_(Th/Ts)均随剂量增加而递增。照射剂量在1～3 Gy范围内,在照射后24、48和72h,小鼠的外周血网织红细胞百分率均随剂量增加而递减;照射剂量在1～7Gy范围内,在照射后6、24和72 h,小鼠的外周血r_(Th/Ts)均随剂量增加而递增。网织红细胞百分率和_(Th/Ts)的剂量-效应关系均满足直线模型。结论:FCM检测网织红细胞百分率和r_(Th/Ts)可成为早期快速、高通量的辐射损伤生物剂量计。OBJECTIVE：To identify the changes of the percentage of reticulocytes and Th-to-Ts ratios（rTh/Ts） from peripheral blood in mice following total body irradiation（TBI） in order to confirm the feasibility of bio-dosimeter about radiation damage.METHODS：Seven to eight weeks-old C57BL/6 mice were treated with TBI at doses ranging from 1 Gy to 7Gy.Peripheral blood was collected for flow cytometric analysis,percentage of reticulocytes and rTh/Ts） were acquired. RESULTS：Reduced reticulocyte percentages were observed with the doses in the range of 0～7 Gy post-irradiation, minimal reticulocyte percentages were observed at 3 d,and the recovery of reticulocytes was increasingly delayed with higher radiation doses.R_（Th/Ts） increased with doses in the range of 0～7 Gy within 72 h post-irradiation. Percentage of reticulocytes declined with the doses in the range of 1～3 Gy at 24,48 and 72 h post-irradiation.rTh/Ts increased with doses in the range of 1～7 Gy at 6,24 and 72 h after TBI.The dose-response relationships of both reticulocyte percentage and rTh/Ts fitted to the lineal model.CONCLUSION：Percentage of reticulocytes and rTh/Ts ratios analyzed with flow cytometry,could be an early,rapid and high-throughput radiation bio-dosimeter.

关 键 词：辐射 生物剂量计 流式细胞术 网织红细胞 T淋巴细胞亚群 

分 类 号：Q691.5[生物学—生物物理学]