阿苯达唑微球肝动脉灌注对大鼠肝泡状棘球蚴病的治疗作用  

Therapeutic Effect of Hepatic Artery Infusion with Albendazole Microspheres on Hepatic Alveolar Echinococcosis in Rats

作　　者：樊玉祥[1] 任伟新[1] 迪力木拉提.巴吾冬 顾俊鹏[1] 许晓东[1] 张海潇[1] 纪卫政[2] 姜涛[2] 温浩[2] 

机构地区：[1]新疆医科大学第一附属医院介入放射科,乌鲁木齐830054 [2]新疆医科大学第一附属医院新疆包虫病临床研究所,乌鲁木齐8300541

出　　处：《中国寄生虫学与寄生虫病杂志》2011年第6期415-418,共4页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的观察阿苯达唑-PEG6000固体分散体壳聚糖微球经肝动脉灌注治疗大鼠肝泡状棘球蚴病的效果。方法将肝泡状棘球蚴病模型大鼠30只,随机均分为3组,即生理盐水对照组(A)、空白微球组(B)和阿苯达唑微球组(C),A、B和C组分别经肝动脉灌注0.3 ml生理盐水、空白微球2.7 mg/kg(溶于0.3 ml生理盐水)、阿苯达唑-PEG6000固体分散体壳聚糖微球2.7 mg/kg(溶于0.3 ml生理盐水)。于灌注后第1、3、7、14和42天采集大鼠外周静脉血,检测白细胞(WBC)、门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)的值。第42天处死全部大鼠,取肝泡状棘球蚴组织,观察各组病理变化。结果各组大鼠白细胞第1天出现暂时性升高[A组(86.11±19.14)×109/L、B组(117.11±21.76)×109/L和C组(118.11±24.52)×109/L],第7天恢复正常[A组(7.85±6.57)×109/L、B组(11.73±4.85)×109/L和C组(8.49±1.36)×109/L]。B、C组在第3天AST、ALT达到最高值[B组AST(193.15±21.57)U/L、ALT(78.39±9.78)U/L;C组AST(189.91±14.06)U/L,ALT(88.43±9.23)U/L)],与A组的差异均具有统计学意义(P<0.05)。第14天恢复正常水平[B组AST(109.31±15.48)U/L、ALT(47.86±9.49)U/L;C组AST(105.37±8.16)U/L、ALT(49.53±6.75)U/L],与A组的差异均无统计学意义(P>0.05)。第42天病理组织学检查结果显示,A、B组大鼠肝泡状棘球蚴结构基本正常,C组病变以生发层坏死为主。结论阿苯达唑-PEG6000固体分散体壳聚糖微球经肝动脉灌注治疗大鼠肝泡状棘球蚴病有一定疗效。Objective To observe therapeutic effect of hepatic artery infusion of albendazole solid dispersion-chitosan microspheres on hepatic alveolar echinococcosis（HAE） in rats.Methods After the establishment of hepatic alveolar echinococcosis model,30 rats were randomly divided into control group（A）,blank microspheres group（B）,and albendazole microspheres group（C） with 10 rats in each group.0.3 ml normal saline,2.7 mg/kg blank microspheres and 2.7 mg/kg albendazole solid dispersion-chitosan microspheres with 0.3 ml normal saline were injected through hepatic artery of rats in the groups of A,B and C,respectively.At 1 d,3 d,7 d,14 d,and 42 d after injection,venous blood were collected,and white blood cells（WBC）,apartate aminotransferase（AST）,and alanine aminotransferase（ALT）were evaluated.All the rats were sacrificed on 42 d after injection,and HAE pathological changes were observed.Results Transient elevation of white blood cells was observed in all groups at 1 d after infusion [Group A（86.11±19.14）×109/L,B（117.11±21.76）×109/L,C（118.11±24.52）×109/L],at 7 d WBC fell to normal level [A（7.85±6.57）×109/L,B（11.73±4.85）×109/L,C（8.49±1.36）×109/L].In groups B and C,AST,ALT reached their peaks on day 3 after infusion [B：AST（193.15±21.57） U/L,ALT（78.39±9.78） U/L;C：AST（189.91±14.06） U/L,ALT（88.43±9.23） U/L],and decreased to normal level at 14 d after infusion [B：AST（109.31±15.48） U/L,ALT（47.855±9.49） U/L;C：AST（105.37±8.16） U/L,ALT（49.53±6.75）U/L].Histopathological examination at 42 d after infusion showed that in groups A and B structure of the cysts was virtually normal,but in group C most cysts showed necrosis in germinal layer.Conclusion Hepatic artery infusion with albendazole solid dispersion-chitosan microspheres shows certain therapeutic effect on hepatic alveolar echinococcosis in rats.

关 键 词：阿苯达唑 壳聚糖微球 肝泡状棘球蚴病 灌注 

分 类 号：R532.32[医药卫生—内科学]