糖尿病大鼠下颌下腺内水通道蛋白-1和5表达变化及意义  

Experssion of AQP1 and AQP5 in the submandibular glands of diabetic rats

作　　者：崔芳芹[1,4] 贾雪梅[1] 汪渊[2] 黄大可[3] 李报[3] 

机构地区：[1]安徽医科大学组织学与胚胎学教研室 [2]安徽医科大学分子生物学实验室 [3]安徽医科大学形态学中心实验室,合肥230032 [4]蚌埠医学院病理生理学教研室,蚌埠233030

出　　处：《解剖学杂志》2011年第6期743-746,共4页Chinese Journal of Anatomy

基　　金：蚌埠医学院院课题（BYl004）

摘　　要：目的：观察水通道蛋白-1（AQPl）和水通道蛋白-5（AQP5）在糖尿病大鼠下颌下腺内表达变化，探讨糖尿病患者口渴症状的发生机制。方法：SD大鼠随机分为对照组、糖尿病组、治疗组。取大鼠下颌下腺，分别进行H-E染色、免疫组织化学显色（SP法）和计算机图像分析。结果：对照组和治疗组的血糖分别与糖尿病组血糖比较，均有差异；与对照组比较，糖尿病组腺泡轻度萎缩，细胞排列紊乱，导管数目减少，直径变小；糖尿病组AQPl和AQP5表达下降；治疗组AQPl和AQP5表达较糖尿病组增加；对照组与糖尿病组大鼠下颌下腺AQPl、AQP5的MOD值比较，均有差异；糖尿病组与治疗组AQPl、AQP5的MOD值比较，均有差异性。结论：糖尿病大鼠下颌下腺内AQPl和AOP5的表达减少，为进一步探讨糖尿病下颌下腺分泌功能降低的发病机制提供形态学依据。Objective： To investigate the expression and significance of AQP1 and AQP5 in the submandibular glands of diabetic rats and further explore the mechanism of diabetic thirst symptom. Methods： Sprague Dawley rats were randomly divided into a control group, DM group and DM-I group. After two months, all rats were sacrificed and the submandibular glands were taken for observing the results using HE staining, immunohistochemistry staining and image analysis by computer. Results： The blood glucose of the control group and DM-I group was compared with that of DM group, and the differences were significant. In comparison with the control group, the acinus was mildly atrophied in DM group; the aeinar cells were arranged irregularly; The number and diameter of the tubule was induced. The result of immunohistoehemisty： Compared with the control group, the expression of AQP1 and AQP5 was reduced in DM group; compared with DM group, the expression of AQP1 and AQP5 was increased in DM-I group. Computer imaging analysis ： the MOD of AQP1 and AQP5 in the control group was compared with that in DM group, and the differences were significant; the MOD of AQP1 and AQP5 in DM-I group was compared with that in DM group, and the differences were significant. Conclusion： The expression of AQP1 and AQP5 was reduced in the submandibular glands of the diabetic rats, and the morphological evidence was further provided for lower secretory function of the submandibular gland in diabetic rats.

关 键 词：糖尿病 水通道蛋白-1 水通道蛋白-5 下颌下腺 大鼠 

分 类 号：R977.1[医药卫生—药品]