雌激素对大鼠胸腺细胞凋亡及Bcl-2、Bax表达的影响  

Effects of estrogen on apoptosis and expression of Bcl-2 and Bax in rat thymus

作　　者：李雅娜[1] 孙研[2] 崔春红[3] 殷彦君[1] 

机构地区：[1]滨州医学院组织学与胚胎学教研室,烟台264003 [2]烟台市烟台山医院,烟台264000 [3]山东万杰医学院组织学与胚胎学教研室,淄博255213

出　　处：《解剖学杂志》2011年第6期747-750,F0002,共5页Chinese Journal of Anatomy

基　　金：山东省教育厅科技计划基金资助（J03K12）

摘　　要：目的：探讨苯甲酸雌二醇对大鼠胸腺Bcl-2和Bax表达及细胞凋亡的影响及其机制。方法：雌性大鼠行卵巢切除术，给予苯甲酸雌二醇后，观察胸腺指数的变化，Hochest33342荧光染色及透射电镜标本观察胸腺细胞凋亡情况，免疫组织化学检测胸腺组织中Bcl一2和Bax的表达情况，原位杂交技术检测Bcl一2、BaxmRNA的表达情况。结果：双侧卵巢切除组大鼠胸腺指数较假手术组增加，双侧卵巢切除＋雌激素组大鼠胸腺指数较双侧卵巢切除组减小；假手术组和双侧卵巢切除组大鼠胸腺组织中以正常胸腺细胞为主，偶见凋亡细胞或凋亡小体，双侧卵巢切除＋雌激素组可见较多凋亡细胞和凋亡小体；双侧卵巢切除＋雌激素组大鼠胸腺组织中Bcl-2表达较双侧卵巢切除组和假手术组增高明显降低，而Pax表达呈现相反趋势；Bcl-2mRNA、BaxmRNA的表达与Bcl-2、Bax的表达呈一致性。结论：雌激素可以降低大鼠胸腺指数，抑制胸腺组织中Bcb2的表达，促进Bax的表达，从而诱导大鼠胸腺细胞凋亡，促进雌性大鼠胸腺退化。Objective： To explore the effects of estrogen on the apoptosis and the expression of Bcl-2 and Bax in rat thymus. Methods： The rats performed with ovariectomy were injected with estradiol benzoate. Thymus was obtained 7 days after the injection. Thymic indexes were measured. Apoptosis of the thymus was detected after Hochest 33342 staining and examined under an electron microscope. The expression of Bcl-2 and Bax in the thymus was detected with a immunohistochemical method. The expression of Bcl-2 mRNA and Bax mRNA in the thymus was detected by in situ hybridization. The test was taken in statistical treatment. Results： The thymus quality index in ovariectomy group was higher than that in the model control group. The thymus quality index of rats injected with estradiol benzoate was reduced respectively. Apoptotic cells and apoptotic bodies were found in the thymus in the groups treated with estradiol benzoate. The expression of Bcl-2 protein in rats injected with estradiol benzoate was lower than that in the control group, and Bax was higher. The expression of Bcl- 2 mRNA and Bax mRNA in the thymus showed consistency with the expression of Bcl-2 and Bax. Conclusion： Estradiol benzoate may increase mass index of thymus, accelerate the degradation of the thymus, induce the apoptosis of the thymus, restrain the exoression of Bcl-2 orotein and oromote the exoression of Bax orotein in the thymus of rats.

关 键 词：雌激素 胸腺 细胞凋亡 BCL-2 BAX 

分 类 号：R542.2[医药卫生—心血管疾病]