小鼠实验性自身免疫脑脊髓炎模型中T细胞免疫球蛋白黏蛋白分子一3的表达及临床意义分析  

Expression and clinical analysis of Tim-3 in experimental encephalomyelitis in mice

作　　者：刘页玲[1] 尹春华[2] 朱清仙[3] 

机构地区：[1]井冈山大学医学院解剖学与组织学胚胎学教研室,吉安343000 [2]2南昌大学第一附属医院妇产科 [3]南昌大学医学院组织学与胚胎学教研室,南昌330006

出　　处：《解剖学杂志》2011年第6期774-777,共4页Chinese Journal of Anatomy

摘　　要：目的：分析小鼠实验性自身免疫脑脊髓炎（EAE）模型中神经系统及引流淋巴结T细胞免疫球蛋白黏蛋白分子（Tim-3）的表达，同时监测阻断该分子功能后对EAE小鼠临床评分的影响。方法：建立小鼠EAE模型，在规定时间点处死小鼠后获取脑部组织及免疫部位引流淋巴结。应用RT-PCR检测脑组织中Tim-3mRNA水平且使用流式细胞术检测引流淋巴结Tim-3阳性细胞。另外，EAE模型构建后分别使用Tim-3封闭抗体或PBS给予治疗，记录实验组及对照组小鼠临床评分。结果：与正常小鼠相比，EAE模型中脑组织Tim-3mRNA表达增高，且引流淋巴结中Tim-3阳性的细胞数也增多。此外，使用Tim-3封闭抗体治疗的小鼠和PBS治疗的相比，明显加重EAE小鼠临床症状。结论：Tim-3分子通路在EAE模型中表达增高，该分子有望成为EAE模型新的治疗靶点。Objective： To analyze the expression of T cell immunoglobulin and mucin domain containing molecules-3 （Tim-3） in central nervous system and draining lymph node in the model of mouse experimental autoimmune encephalomyelitis （EAE）. Meanwhile, to detect the influence of clinical score to EAE mice by blocking Tim-3 signal. Methods： Mouse EAE model was performed and the mice were sacrificed in limited time. Some brain tissues and immune draining lymph nodes were harvested. The levels of Tim-3 mRNA in brain tissues were detected by RT-PCR and the proportion of Tim-3 positive cells in draining lymph node was analyzed by flow cytometry. Moreover, EAE mice were treated with Tim-3 blocking antibody or PBS and the clinical score was recorded. Results： The level of Tim-3 mRNA was increased in brain tissues of EAE mice and Tim-3 positive cells was also enhanced in the draining lymph node, compared to normal mice. Furthermore, the clinical symptom was significantly aggravated in EAE mice treated with Tim-3 blocking antibody, compared to PBS treatment. Conclusion ： The expression of Tim-3 pathway is increased in mouse EAE model and the Tim-3 molecule may be a new target for EAE treatment.

关 键 词：实验性自身免疫脑脊髓炎 T细胞免疫球蛋白黏蛋白分子一3 脑 引流淋巴结 临床评分 

分 类 号：R277.7[医药卫生—中医学]