CO中毒小鼠脑内血管内皮系统Ang-1、Ang-2和Tie-2表达的动态研究  

Dynamic study on Ang-1,Ang-2 and Tie-2 expressions of endothelial system in mouse brain after carbon monoxide poisoning

作　　者：王晓婷[1] 张志清[2] 王晓红[2] 张静[1] 孙利丽[1] 汪怡新[1] 王苏平[2] 

机构地区：[1]大连市友谊医院老年病科,辽宁大连116001 [2]大连市中心医院神经内科,辽宁大连116033

出　　处：《大连医科大学学报》2011年第6期546-550,共5页Journal of Dalian Medical University

摘　　要：[目的]观察CO中毒小鼠脑海马区血管内皮系统Ang-1、Ang-2和Tie-2表达的变化规律,探讨CO中毒对小鼠脑血管内皮系统的影响。[方法]采用逆转录聚合酶链反应(RT-PCR)测定空气对照组和CO中毒组小鼠(各48只)在不同时间点脑海马区Ang-1、Ang-2及Tie-2的mRNA相对表达量。[结果]与对照组比较,CO中毒后脑血管内皮系统Ang-1、Ang-1和Tie-2 mRNA表达均呈双峰性增高,双峰分别在CO中毒后3 d(7.02±0.11、7.67±0.22和8.73±0.07,P<0.05)和7 d(6.60±0.367、.99±0.06和7.69±0.05,P<0.05)。CO组的Ang-2/Ang-1比值在6 h达到第一高峰(1.55±0.07,与对照组比较P<0.05)2,d到达低谷,随后比值缓慢上升于第7天达到第二个高峰(1.24±0.10,与对照组比较P<0.05),14 d恢复正常。[结论]CO中毒后,小鼠脑内血管内皮Ang/Tie-2系统mRNA水平表达呈双峰性增高,第二个高峰可能与迟发性脑病相关。[ Objective] To detect dynamic change of mRNA expressions of Ang - 1, Ang -2 and Tie -2 to reveal the effect of carbon monoxide （CO） on endothelial system of brain. [ Methods] RT- PCR was used to detect mRNA dynamic change of Ang - 1, Ang - 2 and Tie - 2 in the brains of mice in CO poisoning group and control group at different time points. [ Results ] Comparing with control group, after CO poisoning, Ang - 1, Ang - 2 and Tie - 2 mRNA levels showed two peaks, respectively in3 day （7.02＋0.11, 7.67＋0.22 and 8.73 ~0.07, P〈0.05） and7 day （6.60＋0.36, 7.99 ＋ 0.06 and 7.69 ＋ 0.05, P 〈 0.05 ）. The ratio of Ang - 2 to Ang - 1 in CO poisoning group reached the peak at 6 h （ 1.55 ~ 0.07, compared with control group P 〈 0.05 ）, decreased bottom at 2 day, raised slowly to peak at 7 day （ 1.24 ~ 0.10, compared with control group P 〈 0.05 ） , and then decreased to normal at 14 day. [ Conclusions ] The expressions of Ang - 1, Ang - 2 and Tie - 2 mRNA in endothelial system of mouse brain after CO poisoning have two peaks and the sec- ond peak might be related to delayed encephalopathy.

关 键 词：CO中毒 血管内皮系统 ANG-1 Ang-2 TIE-2 

分 类 号：R595.1[医药卫生—内科学]