脉冲电磁场对大鼠破骨细胞功能分子的影响  

Effect of pulsed electromagnetic fields on rat osteoclasts

作　　者：黄晶晶[1] 杨福军[1] 赵吉[1] 孙元明[1] 刘晓秋[1] 沈秀[1] 赵阿津[1] 

机构地区：[1]北京协和医学院,中国医学科学院放射医学研究所,天津300192

出　　处：《中国骨质疏松杂志》2011年第12期1041-1044,共4页Chinese Journal of Osteoporosis

基　　金：天津市应用基础及前沿技术研究计划(08JCYBJC08500);国家自然基金(30970867);北京协和医学院研究生创新基金项目(院1123);中国医学科学院放射医学研究所所基金(ST1108)

摘　　要：目的研究脉冲电磁场(puslsed electromagnetic fields,PEMFs)对大鼠破骨细胞的作用。方法取10w龄雌性SD大鼠随机分为三组:PEMFs组、Control组和Sham组;其中PEMFs组、Control组进行双侧卵巢切除手术,Sham组不切除卵巢。术后12w对PEMFs组大鼠进行PEMFs作用(70Hz,2mT),Control组和Sham组不进行PEMFs作用。作用结束后,取大鼠股骨骨髓,进行体外破骨细胞诱导培养。7天后分别进行破骨细胞的抗酒石酸酸性磷酸酶(Tartrate-resistant acid phosphatase,TRAP)检测和整合素αvβ3、NF-κB受体激活子(receptor activator of NF-κB,RANK)、组织蛋白酶K(cathepsin K)免疫荧光染色检测。结果经PEMFs作用后,TRAP检测显示破骨细胞形态;免疫荧光染色显示阳性破骨细胞也明显减少。结论 PEMFs对破骨细胞功能分子的表达有抑制作用,提示PEMFs作用可以抑制SD大鼠破骨细胞的骨吸收活性。Objective To study the effect of pulsed electromagnetic fields （PEMFs） on rat osteoclast rats. Methods Tenweekold female SD rats were randomly divided into three groups： PEMFs group, CONTROL group, and Sham group. The rats of PEMFs group and CONTROL group were ovariectomized bilaterally. The rats of Sham group were shamovariectomized. The rats of PEMFs group were exposed to PEMFs 12 weeks after ovariectomy. However, the rats of the other groups were not treated with PEMFs. Femoral bone marrow of SD rats was collected after PEMFs. The osteoclasts were induced and cultured in vitro. Tartrateresistant acid phosphatase （TRAP） was measured after 7 days. Immunofluorescence stainings of integrin o%[33, receptor activator of NFKB （RANK） , and cathepsin K were also measured respectively. Results After exposure to PEMFs, the TRAP measurement showed the morphology of the osteoclast. The immunofluorescence staining showed that positive osteoclasts were significantly reduced. Conclusion PEMFs has inhibitive effect on osteoclasts and can inhibit the bone resorbing activity of the osteoclast in SD rats.

关 键 词：脉冲电磁场 去卵巢大鼠 破骨细胞 

分 类 号：R454.1[医药卫生—治疗学] R543[医药卫生—临床医学]