鱼腥草对HIV假病毒作用的初步研究  

Preliminary study of the effect of houttuynia cordata thunb on pseudotyped HIV

作　　者：李文胜[1] 石秀兰[2] 李敏[3] 赵国强[3] 

机构地区：[1]郑州大学第一附属医院检验科,河南郑州450052 [2]郑州市儿童医院检验科,河南郑州450053 [3]郑州大学基础医学院微生物与免疫教研室,河南郑州450001

出　　处：《河南医学研究》2011年第4期395-397,共3页Henan Medical Research

基　　金：河南省医学科技攻关基金资助项目(2008002002)

摘　　要：目的:观察鱼腥草体外对HIV假病毒的作用。方法:鱼腥草给予大鼠灌胃,采血制备含药血清;HIV假病毒载体pNL4-3 Luc R-E-和质粒phRL-SV40瞬时转染HEK293T细胞;含药血清刺激各组细胞,培养24 h后观察荧光素酶活性及检测P24蛋白含量。结果:实验组的荧光素酶值和P 24蛋白含量均低于对照组,差异有统计学意义(P<0.01)。结论:鱼腥草对HIV假病毒复制具有一定的抑制作用,推测其具有潜在的抗艾滋功效。Objective： This study was designed to investigate the effect of Houttuynia cordata thunb on pseudotyped HIV in vitro. Methods： The adult wistar rats were administered intragastrically,and their blood was obtained and the separated serum were termed as medicated serum.Transient transfection was used to co-transfect the pNL4-3 Luc R-E-vectorand phRL-SV40 vector into HEK293T cells.Then the cells were stimulated by medicated serum for 24h to analyze luciferase activity and detect content of P24. Results： The level of luciferase activity and P24 protein of the experimental group were both remarkably lower than that of control（P0.01）. Conclusion： Houttuynia cordata Thunb may have some inhibitory effect on HIV-1 replication and were thus presumed to have the potential anti-AIDS effect.

关 键 词：鱼腥草 HIV假病毒 荧光素酶活性 P24蛋白 HEK293T细胞 

分 类 号：R512.91[医药卫生—内科学]