罗格列酮对大鼠骨密度和骨微结构的影响  

Effect of rosiglitazone on bone mineral density and bone microstructure in rats

作　　者：屈晶晶[1] 盛辉[1] 王睿[1] 王广宇[1] 曲伸[1] 吴国亭[1] 

机构地区：[1]同济大学附属第十人民医院内分泌科,上海200072

出　　处：《同济大学学报（医学版）》2011年第6期21-25,共5页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金(30900698);上海市浦江人才计划(09PJ1410300)

摘　　要：目的研究罗格列酮对去势和不去势大鼠骨密度和骨微结构的影响。方法取32只6月龄SD雌性大鼠随机分为两组,双侧卵巢摘除或仅切除双侧卵巢周围少量脂肪组织,术后三周将全部大鼠随机分为四组,每组8只。sham(R)组和OVX(R)组以罗格列酮每日2 mg/kg溶解于1 m1生理盐水灌胃,Sham(NS)组和OVX(NS)以生理盐水每日1 ml灌胃8周,观察大鼠股骨和腰椎骨密度、骨微结构等指标。结果去势组腰椎骨密度明显降低,OVX(NS)组和OVX(R)组较sham(NS)组分别降低8.5%和14%,OVX(R)组较sham(R)组降低9.1%。OVx(R)组股骨和腰椎骨小梁骨量较sham(NS)组分别减少41.3%和87.5%,较sham(R)组减少37.9%和78.9%。OVX(R)组股骨和腰椎骨小梁厚度较sham(NS)组分别减少11.8%和41.4%,较sham(R)组减少11.8%和33.3%。结论罗格列酮可使大鼠骨密度降低,骨小梁骨量减少,在雌激素缺乏时,罗格列酮对大鼠骨骼破坏加倍,骨密度显著降低,骨微结构明显受损。Objective To investigate the effects of rosiglitazone on bone mineral density and bone microstructure in rats.Methods Thirty two SD female rats aged 6 months were ovariectomized or sham operated.After three weeks,they are randomly assigned into four groups（n = 8 in each）：the sham（R） group and the OVX（R） group received intragastric administration of rosiglitazone 2 mg for 8 weeks,and the sham（NS） group and the OVX（NS） group received intragastric administration of normal saline（1 ml ？ day^（-1）） for 8 weeks.Bone mineral density（BMD） of femur and lumbar were determined by dual-energy X-ray absorptiometry（DXA）.Bone microstructure including trabecular bone volume and trabecular thickness were measured by micro-CT.Results Compared with sham （NS） group,the BMD of lumbar of OVX（NS） group and the OVX（R） group declined by 8.5%and 14%,respectively.The OVX（R） group declined by 9.1%compared with group sham（R）. Compared with the sham（NS） group,the trabecular volume of femur and lumbar in OVX（R） group decreased by 41.3%and 87.5%;while the OVX（R） group decreased by 37.9%and 78.9% compared with the sham（R） group.In addition,the trabecular thickness of femur and lumbar of rats of the OVX（R） group is lower than the sham（NS） group,by 11.8%and 41.4%,while they declined by 11.8%and 33.3%compared with the sham（R） group.Conclusion Rosiglitazone may decrease the BMD and trabecular volume of rats,particularly for the ovariectomized rats.

关 键 词：罗格列酮 骨密度 骨小梁 大鼠 

分 类 号：R58[医药卫生—内分泌]