槲皮素和17-AAG对大鼠外周血淋巴细胞辐射敏感性的影响研究  

Effect of Quercetin and 17-AAG on Radiosensitivity of Rat Peripheral Blood Lymphocyte

作　　者：褚薛刚[1] 洪承皎[1] 张保国[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院,江苏省放射医学与防护重点实验室,江苏苏州215123

出　　处：《辐射防护》2012年第1期28-33,共6页Radiation Protection

基　　金：国家自然科学基金资助项目(10275083)

摘　　要：为研究槲皮素和17-AAG对大鼠外周血淋巴细胞增殖与辐射敏感性的的影响,采用CCK-8试验检测槲皮素和17-AAG对体外培养的大鼠外周血淋巴细胞的毒性作用和它们对淋巴细胞辐射敏感性的影响。结果表明,槲皮素对体外培养的大鼠外周血淋巴细胞有一定的保护作用,保护作用与药物浓度和作用时间呈明显负相关。17-AAG对体外培养的大鼠外周血淋巴细胞具有一定的增殖抑制作用,与药物的浓度和作用时间成正相关。且槲皮素和17-AAG对照射后大鼠外周血淋巴细胞无明显的增殖抑制或保护作用。To investigate the effect of quercetin and 17-AAG on proliferation and on radiosensitivity of blood lymphocyte cells.CCK-8 assay is performed to evaluate the cytotoxicity of Quercetin on proliferation of blood lymphocyte cells.CCK-8 assay employed to observe its effects on the radiosensitivity of the cells quantified by calculating the sensitive enhancement ratio（SER）.CCK-8 results showed that the inhibition of Quercetin on the cells was the dose-dependent and time-dependent,and the results of assay showed the inhibition of 17-AAG on blood lymphocyte cells was the dose-dependent and timedependent.The study showed that Quercetin and 17-AAG have no effect on the radiosensitivity of the blood lymphocyte cells.

关 键 词：槲皮素 17-AAG 大鼠外周血淋巴细胞 辐射敏感性 细胞增殖 

分 类 号：R144[医药卫生—公共卫生与预防医学]