水曲柳体胚发生过程中不同状态类型外植体的生理生化状态  

Physiological and Biochemical Status of Different-Types of Explants in Somatic Embryogenesis of Fraxinus mandshurica

作　　者：丛建民[1,2] 沈海龙[3,4] 李玉花[1,4] 张鹏[3,4] 杨玲[3,4] 黄剑[3,4] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]白城师范学院生物系,吉林白城137000 [3]东北林业大学林学院,黑龙江哈尔滨150040 [4]东北林业大学,林木遗传育种国家重点实验室,黑龙江哈尔滨150040

出　　处：《华南农业大学学报》2012年第1期48-52,共5页Journal of South China Agricultural University

基　　金：国家自然科学基金(30872008)

摘　　要：以水曲柳Fraxinus mandshurica幼嫩合子胚子叶为外植体诱导水曲柳体胚发生,对该过程中外植体的不同状态类型分别取样,检测这些不同状态类型外植体的多糖含量、可溶性蛋白质含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)活性等生理生化指标,分析不同状态类型外植体生理生化与体胚发生、愈伤组织发生、外植体褐化、外植体生长等方面的关系,以便为分析水曲柳体胚发生机理和改善其体胚发生技术提供依据.研究结果表明,不同状态类型外植体的生理生化状态与体胚发生、愈伤组织发生、外植体褐化和生长发育存在非常有趣的相关关系,值得进一步深入解析.Somatic embryogenesis of Fraxinus mandshurica was conducted by using young zygotic cotyledons as explants. The polysaccharide content, soluble protein content, SOD activity, POD activity, CAT activity and PPO activity were tested for analyzing the relationships between the physiological and biochemical index and the status of somatic embryogenesis, callus induction, explants browning and growth of explants. The results showed that there was interesting correlations between the polysaccharide content, soluble protein content, SOD activity, POD activity, CAT activity and PPO activity and the regeneration of callus and somatic embryos, and explant browning and growth, and it is worth being studied further.

关 键 词：水曲柳 体胚发生 外植体 生理生化指标 

分 类 号：S792.41[农业科学—林木遗传育种]