基因VIb亚型半番鸭源新城疫病毒基因组测序及分析  

Sequencing and Analysis Genome of a VIb Subgenotype of Newcastle Disease Virus Isolated from Semi-Muscovy Duck

作　　者：黄秋芳[1,2] 孙敏华[2] 陈少莺[3] 吕殿红[2] 董嘉文[2] 尚毅[1,2] 吴玄光[1] 胡奇林[2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东省农业科学院兽医研究所,广东省兽医公共卫生公共实验室,广东广州510640 [3]福建省农业科学院畜牧兽医研究所,福建福州350013

出　　处：《华南农业大学学报》2012年第1期106-112,共7页Journal of South China Agricultural University

基　　金：广东省兽医公共卫生公共实验室开放基金(GSKJ090201)

摘　　要：根据GenBank上发布的新城疫病毒基因组序列,设计了9对引物,对从福建福州地区发病半番鸭场分离的新城疫病毒FJ-SMD-03株进行了基因组cDNA扩增测序和分析.结果表明:FJ-SMD-03的基因组序列由15 192 nt组成,在NP基因非编码区比Lasota株多了6个核苷酸ACACTC;基因组由6个ORF组成,即3'-NP-P-M-F-HN-L-5'序列.BLAST结果显示:FJ-SMD-03基因组核苷酸与1.3、carrier-pigeon/Guangdong/2008、pigeon/Italy/1166/00、AV324/96和dove/Italy/2736/00相似性最高,达94.9%～98.5%;与疫苗株Lasota、Mukteswar和B1的相似性最低,为80.7%～83.3%;与我国标准强毒株F48E8及2002年从福建番鸭中分离到的基因VIId亚型强毒株Muscovy duck/China(Fujian)/FP1/02相似性较低,分别为82.5%和87.1%.F蛋白裂解位点的氨基酸序列为112 R-R-Q-K-R-F117,具有强毒株序列特征,进化树分析发现,FJ-SMD-03属于ClassⅡ类基因VIb亚型,证明鸭群中有VIb亚型NDV强毒株;FJ-SMD-03株NDV与1.3、carrier-pigeon/Guangdong/2008和pigeon/Italy/1166/00亲缘关系较近,与carrier-pi-geon/Guangdong/2008形成一个小的进化分支,推测FJ-SMD-03株与carrier-pigeon/Guangdong/2008和pigeon/Italy/1166/00株来源于共同的祖代毒株,可能与鸽携带的病毒有关.Based on the genome sequence of newcastle disease virus （NDV） published in GenBank, nine pairs of gene-specific primers were designed to amplify cDNA of FJ-SMD-03 strain isolated from Semi- Muscovy duck in Fuzhou, Fujian , then sequecing and analysis were performed. The results showed that FJ-SMD-03 was 15 192 nucleotides in length which consisted of six ORFs in the order of 3＇leader-NP-P- M-F-HN-L-5＇trailer and it had six nucleotides longer than Lasota strain in non-coding region of NP gene. The genome shared the highest similarity of the nucleotide with 1.3, carrier-pigeon/Guangdong/2008 ,pi- geon/haly/l166/00,AV324/96 and dove/Italy/2736/00 （94. 9% -98.5% ）, and shared the lowest similarity of the nucleotide with vaccine strain Lasota, Mukteswar and B1 （80. 7% - 83.3% ） , whereas it shared lower similarity of the nucleotide with strain F4sEs and Muscovy duck/China（ Fujian）/FP1/02, Subgenotype VⅡd, velogenic, isolated from Muscovy duck in Fujian during 2002 for 82.5% and 87.1% respectively. The motif of cleavage site region of F gene was ^112R-R-Q-K-R-F^117, which was the sequence signature of virulent strain. Phylogenetic analysis revealed that FJ-SMD-03 belonged to subgenotype VIb, Class Ⅱ, which was the first report of the subgenotype VIb virulent NDV strain isolated from Semi-Mus-covy duck. FJ-SMD-03 was elosely associated with 1.3,carrier-pigeon/Guangdong/2008 and pigeon/Italy/1166/00 and formed a seeondary braneh in the phylogenetic tree with earrier-pigeon/Guangdong/ 2008, which suggested that FJ-SMD-03, earrier-pigeon/Guangdong/2008 and pigeon/Italy/1166/00 came from the same origin ,which might be due to virus carried by pigeons.

关 键 词：新城疫病毒 VIb亚型 基因组 序列测定 遗传分析 

分 类 号：S855.3[农业科学—临床兽医学]