逍遥散类方及其分离组分抗抑郁作用的筛选研究  

Screening Study for Anti-Depression Effect of Xiaoyao Powder Prescriptions and Their Separated Parts

作　　者：贾广成[1,2] 田俊生[1] 周玉枝[1] 李金兵[1,2] 郭晓擎[1,2] 李寅生[1,2] 秦雪梅[1] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006

出　　处：《辽宁中医杂志》2012年第1期11-14,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：科技部国际合作项目(2008DFA30430);国家自然科学基金项目(30901960);山西省科技厅/国际科技合作项目(2008081043);山西大学大型仪器开放测试基金项目

摘　　要：目的:研究比较逍遥散类方及其分离组分的抗抑郁作用,筛选最佳的抗抑郁复方及分离组分。方法:首先,以传统中药复方逍遥散、丹栀逍遥散、柴胡疏肝散水煎剂和阳性药(文拉法辛)为干预药物,采用大鼠强迫游泳实验评价各组药效。其次,将逍遥散、丹栀逍遥散和柴胡疏肝散复方及其分离所得的6个不同极性的组份,采用体外活性筛选实验,考察各复方及其分离组份对大鼠脑突触体摄取5-羟色胺(5-HT)、去甲肾上腺素(NA)和多巴胺(DA)的抑制作用。结果:与空白对照组比较,逍遥散水煎剂组、柴胡疏肝散水煎剂组、文拉法辛组均能够不同程度地缩短大鼠强迫游泳不动时间(P<0.05)。在待测样品的终反应浓度为10μg/mL的条件下,逍遥散水煎剂组X1、逍遥散分离组分X6、X7对大鼠脑突触体摄取单胺类神经递质有明显的抑制作用。结论:逍遥散及其分离组分X6、X7表现出良好的抗抑郁作用,可以作为筛选抗抑郁新药的理想组份。Objective ： To investigate the anti - depression effect of Xiaoyao Powder prescriptions and their isolated components and to screen the best prescription and best part. Methods ： Rats were treated orally with Xiaoyao Powder, Danzhi Xiaoyao Powder, Chaihu Shugan Powder or positive drug （venlafaxine） for two weeks. The FST was conducted 1 h after the last drug treatment to evaluate the anti - depression activity of Xiaoyao Powder prescriptions. Furthermore, the inhibition of the prescriptions and their six separated parts of different polarity on rat brain synaptosomal assimilate 5 - hydroxytryptamine （5 - HT）, noradrenaline （NA） and dopamine （DA） were investigated by in vitro activity screening experiment. Results：After a two -week treatment, Xiaoyao Powder,Chaihu Shugan Powder and the venlafaxine reduced the immobility time significantly （compared to FST with control group, P 〈 0.05 ）. In the condition that the final reaction concentration of the samples are 10μg/mL, Xiaoyao Powder and Xiaoyao Powder separated parts X6, X7 could inhibit rat brain synaptosomal to assimilate 5 - hydroxytryptamine （5 - HT）, noradrenaline （NA） and dopamine （DA） significantly. Conclusion：It is a suggestion that Xiaoyao Powder and its isolated parts of Xs, X7 had the favorable anti - depression effect and they could be ideal components for screening novel antidepressant drugs.

关 键 词：逍遥散类方 大鼠强迫游泳 分离组分 神经递质 

分 类 号：R285[医药卫生—中药学]