姜黄素抑制人胃癌BGC-823细胞生长并诱导其凋亡的机制研究  

Introduction of Mechanism on Human Gastric Cancer Cell Line BGC-823 Growth and Inducing Apoptosis

作　　者：覃红斌[1,2] 高嗣法[2,3] 江莹[2,3] 陈思涵[2,3] 甘华文[2,3] 张洁[2] 张玲[2] 魏蕾[2] 张京伟[4] 

机构地区：[1]湖北民族学院医学院,湖北恩施445000 [2]武汉大学基础医学院,湖北武汉430000 [3]武汉大学人民医院,湖北武汉430071 [4]武汉大学中南医院,湖北武汉430000

出　　处：《辽宁中医杂志》2012年第1期23-25,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：湖北省自然科学基金(2008CDB222)

摘　　要：目的:探讨姜黄素抑制人胃癌BGC-823细胞生长并促人胃癌BGC-823细胞凋亡的生物学作用及其调控机制。方法:常规体外培养对数生长期胃癌BGC-823细胞,细胞分为对照组,低、中、高姜黄素处理组四组,姜黄素浓度分别为0mg/L5,mg/L1,0mg/L,20mg/L。姜黄素处理24h后,采用甲基噻唑(MTT)比色法及流式细胞仪测定细胞增殖水平及细胞凋亡率;采用免疫组化法测定细胞内Bax、Bcl-2蛋白表达;采用PCR检测Caspase-3的mRNA表达水平。结果:MTT检测显示姜黄素能抑制人胃癌BGC-823细胞増殖,呈现浓度依赖性;流式细胞仪显示姜黄素能有效诱导细胞的凋亡,呈现浓度依赖性,其中20mg/L姜黄素处理24h后细胞凋亡率为48.3%;免疫组化试验表明姜黄素处理使人胃癌BGC-823细胞中Bax表达水平上调,同时Bcl-2蛋白表达水平下调;且细胞中Caspase-3的mRNA表达水平受姜黄素诱导而增高。结论:姜黄素对人胃癌BGC-823细胞的增殖具有明显抑制作用,呈浓度依赖性促进细胞凋亡,这种生物学效应可能与激活Bax蛋白表达、抑制Bcl-2蛋白表达而活化Caspase-3的信号通路有关。该研究为深入探讨姜黄素诱导人胃癌BGC-823细胞凋亡的机制提供了重要依据。Objective：The study was aimed to investigate the effect and mechanism of curcumin induced apoptosis on human gastric cancer cell line BGC - 823. Methods ： Cultured BGC - 823 cells were divided into control, lower dose curcumin, middle close curcumin and higher dose eurcumin groups,and concentration of cureumin of each group were 0mg/L,Smg/L, 10mg/L, 20mg/L respectively. After pretreated with curcumin for 24h, the proliferation level was measured by MTF assay, cell apoptosis level was detected with flow cytometr. Whilst Bax,Bcl -2 protein expression levels were tested by immunohistochemisty assay and Caspase - 3 mRNA expression level was tested by RT - PCR. Results ： MTT assay showed the inhibitory effect of curcumin on proliferation of BGC - 823 cells was dose dependent. Treatment with increasing dose of cureumin in the cells caused a dose - dependent apoptosis by flow cytometry analysis, such as 20mg/L curcumin pretreatment cause 48.3 % cells apoptosis. Immunohistochem- isty assay data indicated that Bax expression level was enhanced in eurcumin treatment BGC - 823 cells, and Bcl - 2 protein expression level was repressed. And RT - PCR indicated that Caspase - 3 mRNA expression was induced by curcumin. Conclusion： The promotion effect of cucumin on apoptosis of BGC - 823 ceils was dose dependent ,which correlated with Bax up - regulation as well as Bcl - 2 down - regulation and Caspase - 3 activation signal transduction pathway. The data offer the clue for further understanding the inhibition mechanism of cucumin on human gastric cancer cell line BGC - 823.

关 键 词：姜黄素 细胞增殖 细胞凋亡 人胃癌BGC-823细胞系 

分 类 号：R285.5[医药卫生—中药学]