龙葵碱对乳腺癌MCF-7细胞微管系统的影响  

Effects of solanine on microtubules system of human breast cancer MCF-7 cells

作　　者：季宇彬[1,2] 刘家源[1,2] 高世勇[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076

出　　处：《中草药》2012年第1期111-114,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30400591);教育部博士点基金项目(200802400001);黑龙江省博士后资助项目(LBHZ09094);黑龙江省研究生创新基金项目(YJSCX2011-199HSD)

摘　　要：目的研究龙葵碱对乳腺癌MCF-7细胞微管系统的影响。方法 MTT法检测龙葵碱对人乳腺癌MCF-7细胞增殖的抑制作用,流式细胞仪分析龙葵碱对MCF-7细胞周期的影响以及细胞内α-微管蛋白及微管相关蛋白(MAP-2)的变化。结果龙葵碱对MCF-7细胞的IC50为22.08μg/mL,能够将MCF-7细胞阻滞于S期;能够增加MCF-7细胞内α-微管蛋白和MAP-2的量。结论龙葵碱通过升高MCF-7细胞内的微管蛋白及MAP-2的表达,将MCF-7细胞阻滞于S期,从而抑制乳腺癌MCF-7细胞的生长。Objective To investigate the effects of solanine on microtubular system in MCF-7 cell line.Methods Proliferation inhibition of MCF-7 cell line was evaluated by MTT assay.Cell cycle of MCF-7 cells was analyzed and the changes of α-tubulin protein and microtubule-associated protein 2（MAP-2） protein were detected by flow cytometry.Results The IC50of MCF-7 cells was 22.08 μg/mL.Solanine could induce MCF-7 cells arrested in S phaseand increase the levels of α-tubulin and MAP-2 in MCF-7 cell line.Conclusion Solanine could inhibit the MCF-7 cell proliferation by increasing α-tubulin and MAP-2 expression and inducing MCF-7 cells arrested in S phase.

关 键 词：龙葵碱 乳腺癌MCF-7细胞 细胞周期 微管蛋白 细胞增殖 

分 类 号：R282.710.5[医药卫生—中药学]