三种玉簪新品种离体快繁技术的研究  

Study on Rapid Propagation in vitro of Hosta plantaginea of Three New Species

作　　者：杨丹[1] 顾德峰[1] 董然[1] 赵和祥[1] 孙唯一 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118 [2]长春市宽城区园林管理处,吉林长春130118

出　　处：《北方园艺》2012年第1期124-126,共3页Northern Horticulture

基　　金：长春市科技计划资助项目(2008245)

摘　　要：以3种玉簪"金头饰"(H.‘Gloden Tiaia’)、"小黄金叶"(H.‘Gloden Cadet’)和"金鹰"(H.‘Gloden Eagle’)的幼嫩花蕾为外植体诱导不定芽,成功的建立了3个品种的离体快繁体系。结果表明:不定芽诱导最佳培养基为MS+6-BA 2.5mg/L+NAA 0.2mg/L;在增殖培养基中,"金头饰"最佳增殖培养基为MS+6-BA 2.0mg/L+NAA 0.5mg/L;"小黄金叶"最佳增殖培养基为MS+6-BA 1.0mg/L+NAA 0.2mg/L;"金鹰"最佳增殖培养基MS+6-BA 4.0mg/L+NAA0.2mg/L;无生长调节剂的MS为"金鹰"的最佳生根培养基,生根率达100%以上;"小黄金叶"在培养基MS+NAA 0.5mg/L中生根率达到100%以上;"金头饰"在培养基MS+NAA 0.3mg/L中生根率也达到100%以上。其移栽成活率均为100%。The young buds of H. ＇Gloden Tiaia , H. ＇Oloden Cadet ＇and H. ＇Gloden Eagle ＇were usecl as me exp^am,s to develop a protocol for adventitious buds induced, The successful establishment of five species of rapid propagation system. The results showed that the best medium for adventitious buds induced was MSq-6-BA 2.5 mg/i.＋ NAA 0. 2 rag/L; In the proliferation culture, the best proliferation medium for H. ＇Gloden Tiaia＇was MS＋ 6-BA 2.0 mg/L. ＋ NAA 0. 5 mg/L; The best proliferation medium for H. ＂Gloden Cadet＇was MSq-G-BA 1.0 mg/Lq-NAA 0. 2 mg/I.; The best proliferation medium for H. ＇Gloden Eagle＇was MSq-6 BA 4. 0 mg/I.q-NAA 0. 2 mg/L. Medium MS as L. ＇ Gloden Eagle ＇ rooting medium, the rooting rate was over 100 % ; H. ＇ Gloden Gadet ＇ in the rooting medium MS ＋ NAA 0.5 mg/L;rooting rate was over 100~/00 ;H. ＇Gloden Tiaia＇in the rooting medium MS 2c NAA 0. 3 rag/1.; rooting rate was over 100G, The transplant survival rate of 100G.

关 键 词：玉簪 不定芽 组织培养 

分 类 号：S682.19[农业科学—观赏园艺]