白细胞介素-1β和肿瘤坏死因子-α在脑外伤大鼠脑水肿组织中的表达及高压氧对其影响的研究  

Expression of 1L-1β and TNF-α in edematous cerebral tissues of rats after traumatic brain injury and the effect of hyperbaric oxygen on its expression

作　　者：何俊德[1] 谢泽宇[2] 陈葆[1] 瞿文军[1] 

机构地区：[1]广州医学院第一附属医院神经外科,510120 [2]汕头大学医学院第二附属医院神经外科

出　　处：《中国实用医刊》2012年第2期1-3,共3页Chinese Journal of Practical Medicine

基　　金：广东省科技计划项目[粤社科字（2009）135号]

摘　　要：目的探讨脑外伤大鼠脑水肿组织中白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的表达变化及不同时间窗高压氧（HBO）治疗对其表达的影响。方法将96只大鼠随机分为：正常对照组（A组）、液压冲击脑外伤模型组（B组）、液压冲击脑外伤模型＋常规治疗组（C组）、液压冲击脑外伤模型＋常规治疗＋HBO治疗组（D组），每组24只；D组模型建立后根据进行HBO治疗的时间点（3、12、24、72h），分为4亚组（D．3h组、D-12h组、D-24h组和D-72h组，每亚组6只），其中B、C、D组分别按照采用大鼠侧位液压冲击构建脑损伤动物模型后进行不同治疗。RT—PCR和免疫组化分别检测脑组织中IL-1β和TNF-α的mRNA表达和蛋白表达情况。结果IL-1β和TNF-αmRNA表达及蛋白表达方面A组〈D-3h组〈D-12h组〈D-24h组〈D-72h组〈C组〈B组（P均〈0．05）。结论IL-1β和TNF-α与脑外伤大鼠脑水肿组织的形成密切相关，HBO可以下调IL-1β和TNF-α的表达，减轻脑水肿，从而改善颅脑损伤大鼠的学习能力，而且这种高压氧治疗的时间宜尽早实施。Objective To study the changes of expression of IL-1β and TNF-α in edematous cerebral tissues of rats after traumatic brain injury and the effect of hyperbaric oxygen（HBO） on the expression. Methods The traumatic brain injury model was established by use of graded lateral fluid percussion in rats. Minty-six rats were randomly divided into 4 groups： control group （group A）, traumatic brain injury group （group B）, traumatic brain injury and conventional therapy group （group C ）, traumatic brain injury and conventional therapy and HBO therapy group（group D）（n =24）. And group D were sub divided into 4 groups ： group D-3 h, group D-12 h, group D-24 h and groups D-72 h that HBO thera- py was administered at 3, 12, 24, 72 h after traumatic brain injury（n =6）. Cerebral IL-1β and TNF-α protein expression and mRNA expression were tested by immunohistochemistry and RT-PCR in each group after therapy. Results The results of the cerebral protein expression and mRNA expression of IL-1β and TNF-α in rats in each group, group A 〈 group D-3 h 〈 group D-12 h 〈 group D-24 h 〈 group D-72 h 〈 group C 〈 group B （ all P 〈 0. 05）. Conclusions IL-1β and TNF-α have a relevant relation- ship with the formation of brain edema after traumatic brain injury. HBO treatment can effectively improve the cognitive dysfunction through reducing the expression of IL-1β and TNF-α after traumatic brain injury especially within 12 h after traumatic brain injury.

关 键 词：脑外伤 高压氧 白细胞介素-1Β 肿瘤坏死因子-Α 

分 类 号：R651.1[医药卫生—外科学]