乙型肝炎病毒核酸载量与乙型肝炎病毒大蛋白的相关性研究  

Study on the correlation between the virus load and hepatitis B virus large surface protein

作　　者：周丽英[1] 蒋理[2] 

机构地区：[1]第二军医大学长征医院南京分院肝脏科,江苏南京210015 [2]南京医科大学第一附属医院临床检验中心,江苏南京210029

出　　处：《中华疾病控制杂志》2012年第1期39-42,共4页Chinese Journal of Disease Control & Prevention

基　　金：江苏省实验诊断学重点实验室基金(XK200731)

摘　　要：目的探讨慢性乙型肝炎e抗原(HBeAg)阴性患者血清中乙型肝炎病毒(hepatitis B virus,HBV)病毒核酸载量与乙型肝炎病毒大蛋白(hepatitis B virus large surface protein,LHBs)之间的相关性,从而探讨LHBs是否可以用作HBeAg阴性乙型肝炎患者病毒复制程度的判定指标。方法采集83例HBeAg阴性慢性乙型肝炎患者血清,采用ELISA进行LHBs检测,全自动免疫分析仪进行HBeAg检测,实时荧光定量PCR方法进行HBV DNA检测。分析HBV DNA无拷贝数组、低拷贝数组及高拷贝数组3组中LHBs的检出率以及HBV DNA不同拷贝数组与LHBs的相关性。结果在HBV DNA无拷贝数组,LHBs的阳性率为6.4%;低拷贝数组,LHBs的阳性率为56.3%,吸光度值与拷贝数对数值呈正相关,但无统计学意义(r=0.25,P=0.362);高拷贝数组,阳性率为95.0%,吸光度值与拷贝数对数值有良好相关性(r=0.90,P<0.001)。结论检测HBeAg阴性慢性乙型肝炎患者血清LHBs,有助于判断患者体内HBV的复制程度,但是否能够作为HBeAg阴性乙型肝炎抗病毒治疗终点的有效判定指标,尚有待进一步探讨。Objective To investigate the correlation between the virus load and hepatitis B virus large surface protein（LHBs） in serum from chronic hepatitis B patients with e antigen negative,so as to evaluate whether LHBs can be used as a marker to determine the replication extent of hepatitis B virus（HBV） in patients with negative HBeAg.Methods Sera were collected from 83 chronic hepatitis B patients with HBeAg negative and ELISA was used for LHBs detection,automatic immune analyzer for HBeAg testing,and realtime fluorescent quantitative PCR method for HBV DNA detection.The correlation between the detection rates of LHBs in three groups（HBV DNA no-copy group,low-copy group and high-copy group） and the copy number of HBV DNA was analyzed.Results In HBV DNA no-copy group,the positive rate of LHBs was 6.4%;in low-copy group,the positive rate was 56.3%,and the logarithm of copy number was positively correlated with the absorbance values（r=0.25,P=0.362）;in high-copy group,the positive rate was 95.0% and the correlation between the logarithm of copy number and the absorbance value was strongly correlated（r=0.90,P0.001）.Conclusions The detection of LHBs in chronic hepatitis B patients with HBeAg-negative contributes to evaluate the replication extent of HBV in patients.However,whether it can be used as an effective marker to guide the antiviral therapy of HBeAg-negative patients remain to be further studied.

关 键 词：肝炎病毒 乙型 病毒包膜蛋白质类 肝炎e抗原 乙型 

分 类 号：R512.62[医药卫生—内科学] R392.116[医药卫生—临床医学]