水杨酸及其衍生物对溶菌酶光敏损伤的保护作用分析  

Analysis of Protective Action of Salicylic Acid and Its Ramification on Photooxidation Damaged to Lysozyme

作　　者：鲍小丹[1] 马建华[1] 

机构地区：[1]集美大学生物工程学院,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2012年第1期20-25,共6页Journal of Jimei University：Natural Science

基　　金：福建省自然科学基金资助项目(2009J01032);厦门市集美区科技计划项目(350211Z20092C01);集美大学创新研究团队专项基金资助项目(2010A007)

摘　　要：利用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),研究了365 nm紫外照射引发蒽醌-2-磺酸钠(AQS)、溶菌酶(Lysozyme)和水杨酸及其衍生物(水杨醛、水杨酸甲酯)水溶液体系的光化学反应效应及影响因子.结果表明:AQS能够造成Lyso的光敏损伤,且损伤程度随AQS浓度增加及光照时间的延长而加剧;水杨酸及其衍生物对Lyso的光敏损伤具有显著的保护作用,其保护效果与水杨酸及其衍生物的浓度呈正相关;以抗坏血酸(Vc)作为对照,相同条件下,其保护效果依次为:水杨酸≈水杨醛>Vc>水杨酸甲酯.The photochemical reactions and their influence between anthraquinone-2-sulfonic acid （ AQS）, lysozyme, salicylic acid and its ramification by irradiation under 365 nm were investigated using so- dium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. The results showed that lysozyme was photooxidated by AQS, and the damage was dependent on the concentration of AQS as well as the UV treat- ment time. Salicylic acid and its ramification could significantly protect the photooxidation of lysozyme, and the protective effect had positive correlation with their concentration. Compared with ascorbic acid （Vc）, the efficiency of protection was successively as follows, salicylic acid ≈ salicyl aldehyde 〉 Vc 〉 methyl salicy- late.

关 键 词：蒽醌-2-磺酸钠 溶菌酶 光敏损伤 SDS—PAGE 水杨酸 衍生物 

分 类 号：O625.31[理学—有机化学]