蜕皮甾酮联合骨髓间充质干细胞促进烫伤皮肤愈合的实验研究  

Effects of ecdysterone combined with mesenchymal stem cells on wound healing of cutis in rabbits

作　　者：张子良[1] 吴旭[1] 张军花[2] 张元中[1] 冯长江[1] 李军[1] 吴霞[1] 王磊[3] 

机构地区：[1]南方医科大学南方医院胸心外科 [2]南方医科大学南方医院麻醉科 [3]南方医科大学南方医院创伤骨科

出　　处：《实用医学杂志》2012年第1期52-54,共3页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2008B010600006)

摘　　要：目的:探讨蜕皮甾酮(EDS)联合骨髓间充质干细胞(MSCs)促进家兔烫伤皮肤愈合的作用及可能的机制。方法:家兔18只,每只4个创面,随机标记设为空白对照组(A组)、EDS组(B组)、MSCs(C组)、EDS+MSCs(D组)。烫伤后5、10、15d各处死6只家兔,计算创面愈合率,观察组织病理学指标。结果:烫伤后5d,B组创面愈合率显著高于A组,B、D组炎性细胞浸润度显著低于A组。烫伤后10d,各组创面愈合率、组织病理学评分、炎性细胞浸润度之间差异均无显著性。烫伤后15d,B、C、D组创面愈合率、组织病理学评分均高于A组。结论:EDS联合MSCs对家兔烫伤皮肤有促愈合作用。Objective To investigate the effects and the mechanism of ecdysterone （EDS） combined with mesenchymal stem ceils （MSCs） on wound healing of spot scald cuffs in rabbits. Methods There were 18 rabbits, each has 4 scald spots on cutis that labeled randomly into Group A, B, C, and D. Group A was blank control group, Group B was treated by EDS, Group C by MSCs, and Group D by MSCs ＋ EDS. 18 rabbits were sacrificed to obtain the histological specimen at day 5, 10, and 15 （each 6） after modeling. The histopathological indexes were observed and the wound healing rates were calculated. Results At day 5 after modeling, the wound healing rate of Group B was obviously higher than that of Group A （P 〈 0.05）, the inflammatory cell infiltration of Group B and D were lower than that of Group A （P 〈 0.01）. At day 10 after modeling, there were no significant differences in all indexes among all groups （P 〉 0.05）. At day 15 after modeling, the wound healing rates and histopathological scores of Group B, C, and D were obviously higher than that of Group A （P 〈 0.05）. Conclusion EDS combined with MSCs can improve the wound healing process in rabbits.

关 键 词：间质干细胞 蜕皮甾酮 骨髓间充质干细胞 创伤愈合 烫伤 兔 

分 类 号：R644[医药卫生—外科学]