杂交鹅掌楸体胚发生过程的起源及发育过程  

Study on origin and development of somatic embryos of Liriodendron hybrids

作　　者：陈金慧[1] 张艳娟[1] 李婷婷[1] 王鹏凯[1] 王光萍[1] 施季森[1] 

机构地区：[1]南京林业大学森林资源与环境学院,林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2012年第1期16-20,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金青年项目(30901156);国家林业局“948”引进项目(2009-4-24);江苏高校自然科学基金项目(09KJA220001);江苏省高校优势学科建设工程资助项目

摘　　要：以杂交鹅掌楸体细胞胚为材料,利用细胞学技术研究杂交鹅掌楸体细胞胚的起源及发育过程。结果表明:以杂交鹅掌楸未成熟胚为材料,比较容易形成胚性愈伤组织,胚性愈伤组织细胞结构致密、胚性细胞小、直径相等、细胞质浓厚,细胞内大量积累淀粉等物质,胚性愈伤组织表面的一些核质比大的单细胞发育成单细胞原胚,单细胞原胚有厚壁包围,呈生理隔离状态,后经过细胞分裂建立极性,细胞分裂经过球形胚、心形胚、鱼雷胚最后发育有茎段、根端及V形维管束系统的成熟子叶胚。This research was mainly to discover the origin and development of somatic embryos of Liriodendron hybrids with cellular observation. The results showed that embryogenic callus can be easily induced when used immature zygote embryo as explant. The embryogenic callus structure was tight and compacted and embryogenic cells were small, isodiametric and densely cytoplasmic, with a high nucleoplasmic ratio and small starch grains. Some single-cells from the surface of embryogenic callus can grow into single-cell proembryo with thick cell wall, which show they are independent at the beginning of their lives. The significant character of the somatic embryo was its polarity. Plantlct regenerations were developed from global embryo, heart-shaped embryo, torpedo-shaped embryo and cotyledon embryo, in which the V-shaped vessel vascular bundles, shoot tip meristem and root tip meristem were observed.

关 键 词：杂交鹅掌楸 体细胞胚 发育 

分 类 号：S722[农业科学—林木遗传育种] Q813[农业科学—林学]