粳稻大剑叶角资源的发现及剑叶角度的遗传分析与QTL定位  

Discovery of a Germplasm with Large Flag Leaf Angle and Its Genetic Analysis as well as QTL Mapping in japonica Rice

作　　者：胡文德[1,2] 张红[1] 江建华[1] 王盈盈[1] 孙大运[1] 王晓帅[1] 洪德林[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095 [2]北京奥瑞金种业股份有限公司,北京102206

出　　处：《中国水稻科学》2012年第1期34-42,共9页Chinese Journal of Rice Science

基　　金：国家863计划资助项目(2010AA101300);教育部科技基础条件平台重点资助项目(505005);农业部948计划资助项目(2006-G8[4]-31-1)

摘　　要：利用粳稻保持系863B(P1)与A7444(P2)进行配组,构建了P1、P2、F1、B1(F1/P1)、B2(F2/P2)和F26个世代,并对剑叶角度进行遗传分析。调查了P1与P2及BC1F1世代141个单株SSR标记基因型和剑叶角度,构建该组合的SSR标记连锁图谱并定位剑叶角度的QTL。该连锁图谱由79个多态位点构成,全长441.6cM,相邻标记的平均图距为5.6cM。主基因加多基因的遗传模型分析结果表明,剑叶角度受2对主基因+多基因控制,以主基因遗传为主。单标记分析显示有15个标记与剑叶角度呈极显著相关。利用两种分析软件WinQTLCart 2.5和QTL Network 2.0共同检测到2个控制剑叶角度的QTL(qFLA2、qFLA8)。qFLA2位于RM300-RM145区间,qFLA8位于RM6215-RM8265区间,这两个QTL增效等位基因都来自A7444。Genetic analysis for the flag leaf angle was conducted using six generations of P1 , P2, F1, B1, （F1/P1）, B2 （F2/P2 ） and F2 derived from the cross between 863B（a maintainer line of japonica rice） and A7444 （a germplasm with large flag leaf angle）. Genotypes and phenotypes of flag leaf angle were investigated in 863B （P1）, A7444 （P2） and 141 B1 plants. An SSR genetic linkage map was constructed and QTI.s for flag leaf angle were detected. The genetic map containing 79 information loci was constructed,covering a total distance of 441.6 cM, averaging 5.6 cM between two neighboring loci. Results showed that the trait was controlled by two major genes plus polygene with dominance of major genes. Fifteen markers showed highly significant correlations with flag leaf angle based on single marker regression analysis. Two QTLs, qFLA2 and qFLA8 for flag leaf angle were detected at the intervals of RM300-RM145 and RM6215-RM8265, respectively with WinQTLCart 2.5 and QTL Network 2.0. The positive alleles at the two QTLs were both contributed by A7444.

关 键 词：粳稻 剑叶角度 异交率 遗传分析 数量性状基因座 

分 类 号：S511.22[农业科学—作物学]