利用SRAP分子标记分析47份杂草稻样品遗传多样性  

Genetic Diversity of 47 Weedy Rice with SRAP Markers

作　　者：刘丹[1] 孙健[1] 马殿荣[1] 王嘉宇[1] 唐亮[1] 苗微[1] 陈温福[1] 

机构地区：[1]沈阳农业大学水稻研究所/农业部东北水稻生物学与遗传育种重点实验室/教育部与辽宁省共建北方粳稻遗传育种重点实验室,辽宁沈阳110866

出　　处：《中国水稻科学》2012年第1期70-76,共7页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(30671262);辽宁省自然科学基金资助项目(20102192);水稻生物学国家重点实验室开放课题资助项目(090404)

摘　　要：利用SRAP分子标记分析了采集于杂草稻多发地区的47份样本的遗传多样性,旨在从基因的开放式阅读框区域(ORFs)深入研究杂草稻的遗传背景,为进一步揭示其来源提供依据。结果表明,参试的我国杂草稻材料在110对引物组合中共扩增出845种类型的谱带,多态性谱带590种,多态率为69.82%,平均每对引物可以检测到5.4条多态性谱带。说明SRAP分子标记对于杂草稻基因组开放式阅读框区域序列的多态性具有很高的检测效率,适用于遗传多样性研究。我国杂草稻材料Shannon-Weaver多样性指数(I)为0.63,且南北方杂草稻多样性指数(I)相当,分别为0.63和0.62。聚类分析结果表明,南方收集的杂草稻与北方收集杂草稻群体间遗传分化较大,遗传差异明显。程氏指数判定结果也表明杂草稻呈南籼北粳的分布状态。Genetic diversity of 47 weedy rice accessions collected from disaster-prone areas were analyzed with SRAP molecular markers to further reveal the source of weedy rice based on the genetic background of weedy rice at the level of open reading frame. Among 845 DNA bands amplified with 110 pairs of primers 590 were polymorphic, 5.4 bands per pair of primers, accounting for 69.82%. This implied that with a high amplification efficiency at the gene coding region SRAP molecular marker was suitable for genetic diversity analysis. Shannon-Weaver information index of weedy rice in China was 0.63, the diversity index （I） of weedy rice from the north and the south of China were 0.62 and 0.63. On the basis of genetic distance cluster analysis, significant genetic differentiation was detected between weedy rice from the north and the south. Cheng＇s index demonstrated that weedy rice from the south of China could be classified as indica rice and weedy rice from the south as japonica rice.

关 键 词：杂草稻 开放式阅读框 相关序列扩增多态性 遗传多样性 

分 类 号：S511[农业科学—作物学]