稻螟赤眼蜂rDNA特异引物设计及诊断引物在赤眼蜂分子鉴定中的应用  

Design of Specific Primer for Trichogramma japonicum Based on rDNA-ITS2 and Application of Diagnostic Primers in Identification of Four Trichogramma Species

作　　者：郭震[1] 阮长春[1] 臧连生[1] 张帆[2] 靳锋云[3] 

机构地区：[1]吉林农业大学生物防治研究所/天敌昆虫应用技术工程研究中心,吉林长春130118 [2]北京市农林科学院植保环保所,北京100089 [3]吉林省农业技术推广总站,吉林长春130021

出　　处：《中国水稻科学》2012年第1期123-126,共4页Chinese Journal of Rice Science

基　　金：吉林省科技发展计划资助项目(20090560);吉林省留学回国人员科技创新创业择优资助项目;国家科技支撑计划资助项目(2008BADA5B02)

摘　　要：根据稻螟赤眼蜂(Trichogramma japonicum)的rDNA-ITS2序列,利用软件Primer Premier 5.0设计出该寄生蜂的特异引物。通过PCR条件的优化,设计的引物能稳定地扩增出稻螟赤眼蜂的特异性条带,大小为256bp。应用设计的稻螟赤眼蜂特异引物以及报道的松毛虫赤眼蜂(T.dendrolimi)、玉米螟赤眼蜂(T.ostriniae)和螟黄赤眼蜂(T.chilonis)特异引物对赤眼蜂样品进行PCR扩增分析。结果表明,4对特异引物可从单头蜂稳定地扩增出各自蜂种的目的DNA条带,并且分子鉴定结果与形态学鉴定结果完全一致。The species-specific primer for Trichogramma japonicum Ashmead was designed based on its rDNA-ITS2 sequence with Primer Premier 5.0. With improvement of the conditions of PCR, a 256 bp fragment of T. japonicum could be amplified repeatedly with the specificially-designed primer. PCR amplifications of Trichogramma samples were carried out with three pairs of diagnostic primers for T. dendrolimi, T. ostriniae, T. chilonis reported previously, and the designed primer for T. japonicum. The results showed that the specific fragment of each Trichogramna species could be amplified with corresponding specific primers, respectively. Furthermore, the results of molecular identification were consistent with those based on morphological identification of male genitalia .

关 键 词：松毛虫赤眼蜂 玉米螟赤眼蜂 螟黄赤眼蜂 二化螟 分子鉴定 ITS2 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]