羊膜上皮细胞的体外培养及干细胞特性研究  

Human amniotic epithelial cells culture in vitro and the research of Stem cell characteristics

作　　者：耿娟娟[1] 崔勇[1] 邱书奇[2] 

机构地区：[1]广东省医学科学院广东省人民医院耳鼻咽喉-头颈外科,广州市510080 [2]广东省深圳市耳鼻咽喉研究所深圳市龙岗中心医院耳鼻咽喉头颈外科医院,518116

出　　处：《实用医学杂志》2012年第2期172-174,共3页The Journal of Practical Medicine

基　　金：国家自然基金青年项目资助(编号:30801288);教育部留学回国人民科研启动基金(教外司留[2009]1001号);广东省医学科研基金(编号:A2010031)

摘　　要：目的:体外分离培养人羊膜上皮细胞(hAECS)并探讨其生物学特性。方法:取足月产胎儿胎盘,无菌条件下将胎盘的羊膜层与绒毛膜层分离。将洗净的羊膜组织用眼科剪剪成约1mm×1mm大小,加入0.05%的含有0.53mMEDTA4Na的胰酶制备羊膜上皮细胞并进行培养。用免疫荧光、Westernblot等方法检测干细胞相关表面标记物(0CT-4、Nanog、SSEA-4)等的表达情况。结果:根据免疫荧光组织化学,Westernblot的结果显示,羊膜上皮细胞0CT-4、Nanog、ssea-4、Nestin表达阳性。结论:羊膜上皮细胞表达类似胚胎干细胞的表面标志物,可以作为干细胞的新来源。Objective To establish in vitro culture procedure of human amniotic epithelial cells （hAECS） and to explore the biological characteristics. Methods Human placenta were deliveried from third-trimester healthy mothers. The amnion layer was mechanically peeled off of the chorion, washed several times and splitted in 1 mm × 1 mm. The amnion membrane was incubated with 0.05% trypsin containing 0.53 mM EDTA4Na and then cultured. The hAECS were obtained from the amniotic membrane by the digestion of trypsin. The stem cell markers （0CT-4, Nanog,SSEA-4） were examined by the Immunofluorescence, Western. Result The amniotic epithelial ceils express 0CT-4, Nanog, SSEA-4, which is the marker of the stem cells according to immunofluorescence staining and Westemblot results. Conclusion the hAECS express the marker of embryonic stem cell, which means it can be used as a new source of stem cells.

关 键 词：羊膜 上皮细胞 干细胞 生物学特性 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]