微流控细胞芯片LED诱导荧光检测微系统  

Microfluidic Cell Chip Analytical System with LED Induced Fluorescence Detector

作　　者：李栋顺[1,2] 徐溢[1,2,3] 彭金兰[1,2,3] 周桢[1] 甘俊[1,2] 王尧[1,2] 

机构地区：[1]重庆大学化学化工学院,重庆400030 [2]重庆大学新型微纳器件与系统技术国防重点学科实验室,重庆400030 [3]重庆大学微纳系统及新材料技术国际研发中心,重庆400030

出　　处：《高等学校化学学报》2012年第1期49-53,共5页Chemical Journal of Chinese Universities

基　　金：全国博士学位论文作者专项资金(批准号:200941);国家自然科学基金(批准号:20675089);国家科技部"创新药物孵化基地"子课题(批准号:2010ZX09401-306-1-5);重庆大学研究生科技创新基金(批准号:CDJXS10-22-11-39);中央高校基本科研业务费专项资金(批准号:CDJXS1-22-00-05)资助

摘　　要：基于微流控细胞芯片分析技术和微机电系统(MEMS)加工技术,设计制作了阵列式微流控细胞检测芯片,采用自组装的顶窗型光电倍增管(PMT)和信号采集电路采集芯片微管道内流动细胞的荧光信号,构建了一种针对低浓度细胞悬浮液的微流控细胞芯片发光二极管(LED)诱导荧光的快速检测微系统,实现了对低浓度(≤40 Cell/mL)荧光标记的HepG2肝癌细胞悬浮液样本的定量计数和测试,而且在血液细胞共存的条件下,仍可以有效地对血液中少量HepG2肝癌细胞进行荧光计数和测试.整个系统结构简单、操作方便且检测灵敏度较高.Based on micro-electro-mechanical systems technique and microfluidic cell chip analytical metho-dology,a microfluidic cell chip analytical system with light emitting diode（LED） induced fluorescence detector was constructed for monitoring cells in the suspensions with a low concentration below 40 Cell/mL.Cells passed through the micro-channels on the designed microchip,and the target cells labeled by 5-or 6-（N-succinimidyloxycarbonyl）-3′,6′-O,O′-diacetylfluorescein（CFDA SE） were stimulated by the LED to produce fluorescence signals which were collected by a photomultiplier tube from the roof window and changed into electrical signals finally.The HepG2 hepatoma cells labeled by CFDA SE in samples could be detected and counted by this analytical microsystem.The designed microsystem ran effectively with the coexistence of blood cells.The whole microsystem is simple,convenient and high sensitivity.

关 键 词：微流控芯片 HEPG2肝癌细胞 细胞检测 发光二极管(LED)诱导荧光检测微系统 

分 类 号：O657[理学—分析化学]