基于多巴胺自聚合及肝素固定改善钛的血液相容性  

Blood Compatibility Improvement of Titanium via Self-polymerization of Dopamine and Covalent Graft of Heparin

作　　者：谢槟[1] 李贵才[1] 代璐[1] 杨苹[1] 黄楠[1] 

机构地区：[1]西南交通大学材料先进技术教育部重点实验室,成都610031

出　　处：《高等学校化学学报》2012年第1期90-95,共6页Chemical Journal of Chinese Universities

基　　金：中央高校基本科研业务费专项资金(批准号:2010XS32);国家"九七三"计划项目(批准号:2005CB623904);国家自然科学基金(批准号:30870629)资助

摘　　要：利用多巴胺自聚合及肝素固定的方法对纯钛进行表面修饰,以改善其血液相容性.采用水接触角测量、X射线光电子能谱(XPS)和甲苯胺蓝法(TBO)等方法对所修饰的材料进行了表征.采用溶血实验检测了材料的溶血性能,并结合活化部分凝血活酶时间(APTT)测试和血小板黏附实验对所修饰材料的血液相容性进行了评价.结果表明,多巴胺能够在钛表面实现自聚合,肝素可以共价接枝在聚多巴胺层上,经肝素修饰后的材料的表面亲水性显著提高,而且具有较低的溶血率,APTT时间显著延长,血小板的黏附数量和被激活程度也显著降低.因此,纯钛经多巴胺自聚合以及肝素接枝修饰后的血液相容性得到了显著改善,有望成为具有抗凝血功能的新型心血管植入材料.elf-polymerization of dopamine and immobilization of heparin were applied for surface-modification of titanium in order to improve its blood compatibility.Surface modification was developed via self-polymerization of dopamine and covalent graft of heparin.Surface elemental composition was analyzed qualitatively by X-ray photoelectron spectroscopy（XPS）.Surface heparin content was detected by toluidine blue O（TBO）.Surface hydrophilicity and hydrophobicity were characterized by water contact angle measurement.Hemolysis rate test,which was applied for detection ofhemolytic ability,was combined with activated partial thromboplastin time test（APTT） and platelet adhesion test to evaluate blood compatibility.The results showed that self-polymerization of dopamine was achieved on titanium surface.Heparin was successfully immobilized onto polydopamine layer by covalent graft.The heparin-modified surface could result in a significantly improved hydrophilicity,low hemolysis rate,prolonged APTT and a remarkablely decrease both in adhesion amount and activation degree of platelet.The blood compatibility of titanium could be significantly improved by the method of self-polymerization of dopamine and covalent graft of heparin.This study provides potential application for cardiovascular implants materials with anticoagulant properties.

关 键 词：钛 多巴胺 肝素 血液相容性 

分 类 号：O625.3[理学—有机化学]