乙酸和β-羟丁酸对体外培养牛肝细胞脂代谢部分关键酶表达的影响  

Effects of acetic acid and β-hydroxybutyrate on expression of key enzyme genes involved to fatty acid metabolism in bovine hepatocytes cultured in vitro

作　　者：白鸽[1] 许远靖[1] 王冲[1] 孙勇[1] 陈灰[1] 李心慰[1] 王振全[1] 邓清华[1] 王建国[1] 刘国文[1] 周昌芳[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2012年第1期69-72,88,共5页Chinese Journal of Veterinary Science

基　　金：国家"973"计划资助项目(2011CB100800)

摘　　要：以体外原代培养的奶牛肝细胞为模型,添加不同浓度的乙酸(Acetic acid,AcOH)和β-羟丁酸(β-hydroxybu-tyrate,BHBA)共培养24h后,提取细胞总RNA。应用实时荧光定量PCR方法检测脂代谢关键酶长链脂酰辅酶A合成酶1(Long-chain acyl-CoA synthetase-1,ACSL1)、柠檬酸合成酶(Citrate synthase,CS)和乙酰辅酶A羧化酶α(Acetyl coenzyme A carboxylaseα,ACCα)mRNA丰度的变化。结果显示,适当浓度的AcOH能够促进肝细胞脂肪酸活化及氧化途径关键酶ACSL1和CS的转录,而高浓度AcOH能够抑制脂肪酸从头合成途径关键酶ACCα的转录;高浓度BHBA能够抑制肝细胞ACSL1、CS和ACCα的转录。结果表明,血液中适当浓度的AcOH能够促进肝脏脂肪酸氧化并抑制脂肪酸从头合成,高浓度BHBA能够抑制肝脏脂氧化和合成,影响乳脂前体物的供应,进而影响乳脂合成。The objective of this study was to investigate the effects of acetic acid （AcOH） and β-hydroxybutyrate （BHBA） on the transcription levels of key enzymes involved to fatty acid metabolism in the bovine hepatocytes cultured in vitro. The total RNA was isolated from hepatocytes, which were treated with different concentrations of AcOH or BHBA for 24 h. The mRNA expression levels of the key lipid metabolism genes,including long-chain acyl- Co A synthetase-1 （ACSL1）, citrate s ynthase （CS） and acetyl coenzyme A carboxylase a （ACCa）, were determined u- sing fluorescent quantitative real time PCR. The results showed that appropriate concentration of AcOH can promote the transcription of ACSL1 and CS. However, high concentration of AcOH could inhibit ACCa transcription, which inhibit the de nove synthesis of fatty acid in the liver. Furthermore, high concentration of BHBA showed inhibition effects on the ACSL1, CS and ACCa transcription. In summary, the results of this study indicate that appropriate plasma concentration of AcOH promotes lipid oxidation and inhibits the de nove synthesis of fatty acid in the liver. Furthermore,high concentration of BHBA inhibits lipid synthesis and oxidation in the liver, thereby affecting the supply of milk fat precursors, which affects the milk fat synthesis.

关 键 词：乙酸 Β-羟丁酸 肝细胞 脂代谢关键酶 

分 类 号：S852.2[农业科学—基础兽医学]