利用基因芯片技术研究隐性白鸡不同时期肌肉组织相关差异表达基因  

Analysis on differentially expressed genes of muscle tissue in recessive white chicken by microarray

作　　者：栾德琴[1] 常国斌[1] 龚琳琳[1] 周伟[1] 陈丹艳[2] 刘岩[3] 王克华[4] 窦套存[4] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]河海大学水利水电学院水文水资源与水利工程科学国家重点实验室,江苏南京210098 [3]江苏省农业科学院农业资源与环境研究所/数字农业工程技术研究中心,江苏南京210014 [4]中国农业科学院家禽研究所,江苏扬州225003

出　　处：《中国兽医学报》2012年第1期83-88,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972088)

摘　　要：应用包含13 319条鸡基因探针序列的表达谱芯片,对从隐性白鸡不同时期肌肉组织抽提及纯化的cRNA进行芯片杂交,并对基因表达谱进行分析,旨在筛选隐性白鸡不同时期肌肉组织相关差异表达基因,探讨造成不同时期肌肉组织生长发育的分子生物学机理。结果显示,在不同生长时期(2周和12周)共筛选出差异表达基因78条(表达上调34条,表达下调44条),其中已知功能基因39条,主要涉及生长发育、分子代谢、免疫应答、生物合成、细胞通信以及蛋白质合成与分解等相关基因,其中包括一些尚未在GenBank上登陆的序列,推测可能是未知的新基因,它们在鸡生长发育、能量代谢等的过程所起到的作用还需进一步证明。Microarray containing 13 319 probes was used to construct gene expression profile in order to screen differ entially expressed genes of muscle tissue in recessive white chicken and investigate the molecular mechanism related with muscle tissue traits between the two groups. 78 differentially expressed genes,of which 39 are known genes,34 up-regulated,44 down-regulated, being involved in growth, molecular mechanism, immune system process, biosynthetic process, cell communication, protein synthesis and degradation were screened out. Meanwhile, some genes among all these differentially expressed genes that had no annotation in GenBank were screened out; they were presumed to be unknown new genes. The roles that they may play in chicken fat metabolism need to clarify later.

关 键 词：鸡 基因芯片 肌肉组织 基因表达谱 

分 类 号：Q78[生物学—分子生物学] S852[农业科学—基础兽医学]