鉴定牛体外培养胚胎性别的一种新PCR方法及其应用  

Sex identification of bovine embryos in vitro with a new method of PCR

作　　者：刘卓[1] 李纯锦[1] 李万宏[1] 陆晨[1] 侯晓峰[1] 周虚[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2012年第1期140-143,共4页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2011BAD19B02);长春市科技发展计划资助项目(08GH08)

摘　　要：根据已知序列设计了2对引物,分别在体外扩增SRY基因及1个常染色体基因,能同时扩增出2个基因的胚胎为雄性,只能扩增出常染色体基因的胚胎为雌性。通过该方法对50个卵母细胞进行PCR验证,有49个扩增出1条常染色体基因,准确率为98%;鉴定了48枚用普通精液受精所得胚胎及57枚用经SRY抗体处理的精液受精所得胚胎的性别,雌性比例分别为56%和82%。With the development of molecular biology,PCR has been the major method for sex identification of early embryos. PCR is sensitive,rapid,simply to operation that is of a great significant for sex determination of early embryos. Two pairs of primers were designed according to the known gene sequences,SRY gene and an autosomal gene were amplified in vitro. Both genes can be amplified was male,only the autosomal gene which can be amplified was female. 50 oocytes were identificated which by this method. There were 49 oocytes which amplified the autosomal gene. The accuracy rate was 98% . 48 embryos fertilized with normal semen and 57 embryos fertilized with the semen treated by SRY antibody were identified. The female rates were 56% and 82% .

关 键 词：PCR 牛体外培养胚胎 性别鉴定 

分 类 号：S814.7[农业科学—畜牧学]