安络化纤丸联合γ-干扰素治疗小鼠血吸虫性肝纤维化的实验研究  

Experimental Research of Anluohuaxian Pill Plus Interferon-γ on Schistosomal Liver Fibrosis in Mice

作　　者：肖非[1] 马科[1] 黄海燕 艾国[1] 焦云桃[1] 宁琴[1] 黄加权[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染科,武汉430030 [2]湖北省咸宁市第一人民医院病理科,437000

出　　处：《医药导报》2012年第1期1-4,共4页Herald of Medicine

基　　金：湖北省血吸虫病防治项目(XF06c28);湖北省卫生厅血吸虫病防治专项(XF2008-11)

摘　　要：目的比较中成药安络化纤丸、西药γ-干扰素以及二者联合使用对血吸虫性肝纤维化的治疗作用,初步探讨作用机制。方法将50只昆明小鼠随机分为5组,正常对照组10只,其余40只以每只感染日本血吸虫尾蚴40条为入选对象,6周后成功建立小鼠血吸虫性肝纤维化模型。将建模成功的小鼠随机分为4组,每组10只。感染对照组用0.9%氯化钠溶液灌胃,每次0.75 mL。安络化纤丸组用0.9%氯化钠溶液配制混悬液灌胃(安络化纤丸20粒加0.9%氯化钠溶液7.5 mL),每次0.75 mL。γ-干扰素组用灭菌注射用水配制溶液皮下注射(γ-干扰素200万U加注射用水4 mL,每次0.1 mL)。联合治疗组用等量的安络化纤丸和γ-干扰素。上述治疗均为每天1次,疗程8周。正常对照组给予等量0.9%氯化钠溶液灌胃8周。肝组织切片,苏木精-伊红(HE)染色观察病理改变。免疫组化检测肝组织Ⅰ、Ⅲ型胶原及基质金属蛋白酶组织抑制因子-1(TIMP-1)的表达。结果安络化纤丸组、γ干扰素组、联合治疗组、感染对照组血吸虫卵结节直径分别为(460.406 5±36.276 8),(433.927 6±44.209 5),(433.430 0±64.928 4),(533.765 8±88.102 2)μm,与感染对照组比较,各治疗组小鼠肝脏病理损害减轻,血吸虫虫卵结节直径明显减小(P<0.05)。Ⅰ、Ⅲ型胶原及TIMP-1的表达,各组与感染对照组比较均明显下降;联合治疗组表达最低,与单药治疗组比较,差异有统计学意义(P<0.05)。结论安络化纤丸联合γ-干扰素治疗血吸虫性肝纤维化的效果优于单药治疗,其作用机制可能与抑制肝星状细胞激活、减少TIMP-1表达有关。Objective To compare the effects of anluohuaxian pill plus interferon-γ against schistosomal liver fibrosis with anluohuaxian pill used alone. Methods Fifty Kunming mice were divided into 5 groups. Except 10 mice in the normal control, the other 40 were infected with 40 Schistosoma japonicum per mouse. Six weeks later, the model of schistosomal liver fibrosis was successfully induced, and the model mice were randomly divided into 4 groups evenly. ① The model control mice were treated by intragastric administration of 0.75 mL normal saline at each time point;②In the anluohuaxian pill group, mice were i. g. treated with anluohuaxian pill mixed with normal saline at the same volume as above;③In the interferon-γ group, mice were hypodermically injected with 0.1 mL mixture of interferon-γ （2×10^6 U） and pyrogen-free water（4 mL） ;④In the two-drug combination group, mice were treated with half-and-half anluohuaxian pill and interferon-γ one time per day for 8 weeks. By the end of therapy, the liver tissues were taken for pathological examination and expression of type Ⅰ , Ⅲ collagen and TIMP-1 （ tissue inhibitor of metalloproteinases 1 ） was detected. Results The apparently diminished size of schistosomal egg nodules of the anluohuaxian pill group, the interferon-γ group, the two-drug group, the model control were （ 460. 406 5± 36. 276 8 ）, （443. 927 6±44. 209 5 ）, （433. 430 0±64. 928 4 ）, （533. 765 8±88.102 2） μm, respectively. Compared with the model control, the hepatic injury was attenuated in all therapeutic groups with apparently diminished size of schistosomal egg nodules, P〈0.05. Further, the expression of type Ⅰ , Ⅱ collagen as well as TIMP-1 were all conspicuously reduced, especially in the two-drug combined group（P〈0.05）. Conclusion Combination of anluohuaxian pill with interferon-γ shows more effective on treating sehistosomal liver fibrosis, which is possibly associated with inhibition of hepatic stellate cell activation and diminished expressi

关 键 词：安络化纤丸 Γ-干扰素 日本血吸虫 肝纤维化 基质金属蛋白酶组织抑制因子 

分 类 号：R286[医药卫生—中药学] R977.6[医药卫生—中医学]