斑蝥酸钠对人正常肝细胞LO2的体外细胞毒性研究  

Research of Disodium Cantharidinate on In-vitro Cell Toxicity of Normal Human Hepatocyte LO2

作　　者：严晓莺[1] 陈巨鹏[2] 徐德国[1] 赵凤鸣[1] 许冬青[1] 周韬[1] 王明艳[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210046 [2]南京中医药大学附属医院,江苏南京210029

出　　处：《南京中医药大学学报》2012年第1期68-70,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(30973742/C190201);江苏省中医药药效与安全评价重点实验室开放课题(T09019);南京中医药大学基础医学院青年科技创新基金(10jcqn07)

摘　　要：目的探讨抗肿瘤药物斑蝥酸钠注射液(Disodium cantharidinate)对正常人肝细胞(LO2)的体外细胞毒性。方法采用MTT实验、Hoechst-33258荧光染色法和电镜检测。结果斑蝥酸钠作用24h后,与阴性对照组相比0.625、1.25、2.5μg/mL用药组出现不同程度的细胞增殖抑制,抑制率分别为9.1%、17.1%、23.9%;荧光染色提示1.25μg/mL和2.5μg/mL组细胞有形态改变,染色质损伤;电镜结果显示2.5μg/mL用药组出现细胞凋亡。结论抗肿瘤药物斑蝥酸钠有抑制LO2细胞增殖的作用,在一定剂量下能诱导LO2细胞染色质损伤和细胞凋亡。OBJECTIVE To explore in-vitro cell toxicity of anti-tumor drug disodium cantharidinate on normal human hepa tocyte （LO2）. METHODS MTT experiment, Hoechst-33258 fluorescent staining and electron microscope detection were applied. RESULTS Medication groups with dose of 0. 625, 1.25 and 2.5 μg/mL respectively presented different degrees of proliferation inhibition after disodium cantharidinate acted on 24 h, and inhibitory rates were 9.1%, 17.1%and 23.9 % respective ly; Fluorescent staining and electron microscope detection indicated that cells manifested morphological changes, injured chromatin and apoptosis in 2.5μg/mL group. CONCLUSION Anti tumor drug disodium cantharidinate has the inhibitory effect on the proliferation of cell LO2 and can evoke LO2 apoptosis under certain doses.

关 键 词：斑蝥酸钠 肝细胞 毒性 

分 类 号：R285[医药卫生—中药学]