香加皮不同组分多次给药对小鼠肝毒性“量-时-毒”关系研究  

Experimental Study on the "Dose-Time-Toxicity" Relationship of Hepatotoxicity Induced by Different Components from Cortex Periplocae in Mice

作　　者：鲍志烨[1,2] 黄伟[1] 张亚囡[2] 孙蓉[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250355

出　　处：《中国药物警戒》2012年第1期16-19,共4页Chinese Journal of Pharmacovigilance

基　　金：国家973计划中医基础理论专项课题;有毒中药的毒性与功效;证候关系的基础研究(课题编号:2009CB522802)

摘　　要：目的研究香加皮水提组分和醇提组分多次给药致小鼠肝毒性的“量-时-毒”关系。方法取小鼠按不同时间点或不同剂量分组，观察小鼠死亡情况和毒性反应，分别于药后相应时间点检查血清肝功指标和肝脏组织ALT、AST活性，计算肝、肾脏器指数。结果香加皮水提组分和醇提组分对小鼠多次给药肝毒性“量-时-毒”关系研究为：ALT、AST在给药后1天明显升高，以AST为更敏感，一直持续到7天。药后7天之内，水提组分在（0．78～15．0）g·kg。。剂量范围之内、醇提组分在（0．78～6．0）g·kg-1剂量范围之内，与正常组比较，香加皮水提和醇提组分均可造成明显的肝毒性损伤，表现ALT、AST、ALP、TBI升高，ALB降低，肝体和肾体比值有不同程度的增高，呈现明显的量效和时效关系；且醇提组分的肝毒性作用程度〉水提组分。结论多次给小鼠一定剂量的香加皮水提组分和醇提组分均可造成明显的肝损伤，并呈现明显的肝毒性“量-时-毒”关系。Objective To study the ＂dosage-time-toxicity＂ relationship ofhepatotoxicity induced by multiple intragastric administration of water extracted and alcohol extracted components of Cortex Periplocae to mice. Methods Mice are grouped according to different time or dose points, the general condition and toxic effects of mice were observed. The changes of the activity of ALT, AST, etc and liver, kidney index were detected. Results On the first day after administration the hepatotoxicity which displayed with obvious increase of activity in serum ALT, AST, especially the changes of AST were more sensitive. The conditions continued for about 7 days. Compared with the normal group, within 7 days after the administration, water extracted components in（0.78-15.0） g·kg^-1 doses of scope, alcohol extracted components in（0.78-6.0） g·kg^-1 doses of scope, they all cause significant damage to liver, the activity of ALT, AST, ALP, and TBI elevated, while ALB reduced, and liver ratio increased, it shows there were obvious ＂dosage-time-toxicity＂ relations. The degree of hepatotoxicity damage caused by the alcohol extracted composition is more serious than that by the water extracted composition. Conclusion Multiple intragastric administrations of water extracted and alcohol extracted components of Cortex PeripIocae with certain dosage may induce acute hepatotoxical injury in mice and show an obvious ＂dosage-time-toxicity＂ relationship.

关 键 词：香加皮 不同组分 小鼠 肝毒性“量-时-毒”关系 

分 类 号：R285.5[医药卫生—中药学]