膈下逐瘀汤联合环磷酰胺对EAC肝癌小鼠肿瘤间质血管生成的影响  

Experimental study of Gexia-Zhuyu decoction combined with cyclophosphamide on tumor interstitial in rats of EAC liver cancer

作　　者：赵红霞[1] 王莹[2] 赵金茹[3] 王珊丽[4] 李彬彬[1] 宋伟[4] 刘春英[5] 

机构地区：[1]辽宁中医药大学2009硕士研究生,沈阳110023 [2]辽宁中医药大学基础医学院病理教研室,沈阳110023 [3]辽宁中医药大学 实验中心形态实验室,沈阳110023 [4]辽宁中医药大学 2008级硕士研究生 ,沈阳110023 [5]辽宁中医药大学 研究生学院 ,沈阳110023

出　　处：《国际中医中药杂志》2012年第1期25-27,共3页International Journal of Traditional Chinese Medicine

摘　　要：目的探讨膈下逐瘀汤联合环磷酰胺对EAC肝癌模型小鼠的抗癌作用及其机制。方法选EAC肝癌小鼠细胞株，建立肿瘤动物模型后，按完全随机化原则分为空白组、模型组、中药组、CTX组、中药＋CTX组。空白组、模型组给予生理盐水灌胃0．4ml／d；中药组给予膈下逐瘀汤（11g／kg体重）0．4ml/d灌胃：CTX组给予环磷酰胺（2mg／m1）腹腔注射0．4ml／d；中药＋CTX组给予膈下逐瘀汤0．4ml／d灌胃、CTX腹腔注射0．4ml／d。给药10d后，检测各组小鼠的体重、一般状态、光镜下进行形态学观察，电镜下观察肿瘤间质血管生成情况。结果中药＋CTX组可改善小鼠食欲，增加体重（33．60±2．14）g。中药＋CTX组可减少肿瘤间质中毛细血管数量；电镜下可见中药＋CTX组肿瘤组织内新生的毛细血管结构破坏最为严重。结论中药＋CTX组可提高EAC肝癌模型小鼠的生活质量，抑制肿瘤间质毛细血管的生长。Objective To study the anti-cancer effects and mechanism of Gexia-Zhuyu decoction combined with cyclophosphamide on EAC liver cancer mice. Methods Choose EAC liver cancer mice cell lines and production of animal models of tumor. The mice were completely randomized into blank group, model group, the Chinese medicine group, CTX group, Chinese medicine and CTX group. The blank group and model group were given normal saline 0.4 ml/d; traditional Chinese medicine group was administrated with Gexia-Zhuyu decoction （11 g/kg body weight ） 0.4 ml/d gavage; CTX group was given intraperitoneal injection of 2 mg/ml of cyclophosphamide 0.4 ml/d; and traditional Chinese medicine and CTX group was given Gexia Zhuyu decoction 0.4 ml/d orally and CTX intraperitoneal injection of 0.4 ml/d. Ten days after the administration, the body weight of mice and the general condition were tested, and morphology was observed under the light microscope electron microscope mesenchymal tumor angiogenesis situation. Results Gexia-Zhuyu decoction combined with cyclophosphamide in mice could improve appetite, gain weight, and greatly reduce the tumor stroma in the number of capillaries under the electron microscope together with significant pathological morphological changes. Conclusion Gexia-Zhuyu decoction combined with cyclophosphamide had anti-cancer effects which can improve the quality of life and inhibit the growth of tumor in interstitial capillaries.

关 键 词：膈下逐瘀汤 环磷酰胺 肝癌 

分 类 号：R285.5[医药卫生—中药学]