植物次生物质对棉蚜谷胱甘肽S-转移酶和羧酸酯酶活性的诱导作用  

Effects of Secondary Metabolites on Activities of Glutathione S-transferases, Carboxylesterase in Aphid

作　　者：吕敏[1,2] 孙婳婳[3] 王丽红[1] 高希武[1] 

机构地区：[1]中国农业大学昆虫系,北京100193 [2]江苏里下河地区农业科学研究所,江苏扬州225007 [3]南京农业大学植物保护学院,南京210095

出　　处：《中国农学通报》2012年第3期253-256,共4页Chinese Agricultural Science Bulletin

基　　金：公益性行业(农业)科研专项"农药高效安全科学施用技术"(200903033)

摘　　要：为了明确植物次生物质对昆虫解毒酶的影响作用,利用饲喂法和2-十三烷酮熏蒸法研究了植物次生物质2-十三烷酮和槲皮素对棉蚜谷胱甘肽S-转移酶和羧酸酯酶的诱导作用。结果表明,取食含0.1%2-十三烷酮饲料的棉蚜谷胱甘肽S-转移酶的活性最大,取食含0.01%槲皮素的饲料棉蚜羧酸酯酶的活性最大,均与对照差异显著。用2-十三烷酮熏蒸处理24h,棉蚜谷胱甘肽S-转移酶的活性最大,与对照比差异显著。以α-NA和β-NA为底物测定棉蚜羧酸酯酶的活性在处理48h达到最大,分别为对照的2.45和2.53倍,与对照比差异显著。由此可见,饲喂法处理2-十三烷酮对棉蚜谷胱甘肽S-转移酶及槲皮素对棉蚜羧酸酯酶的诱导作用具有显著的剂量效应;亚致死剂量2-十三烷酮熏蒸对棉蚜谷胱甘肽S-转移酶和羧酸酯酶的诱导作用具有显著的时间效应。The aim was to clarify the effects of plant allelochemicals on the detoxification enzyme activities in aphid. The effects of 2-tridecanone and quercetin on the specific activity of glutathione S-transferases （GSTs） and carboxylesterase （CarE） in aphid were investigated by using feeding and fumigation treatments. The results showed that the aphids fed on artificial diet added 0.1% 2-tridecanone and 0.01% quercetin showed the maximum activities of GSTs and CarE, respectively. The maximum activity of GSTs was at 24 h and the difference was significant compared to the control. The maximum activities of CarE measured using α -NA and β -NA as substrates were at 48 h, and the difference were significant compared the control. The activities were increased by 2.45-fold and 2.53-fold respectively compared to the control. Thus, the activities of GSTs and CarE were induced on dosage significantly, by feeding 2-tridecanone and quercetin respectively, and on time significantly by 2-tridecanone fumigation.

关 键 词：棉蚜 2-十三烷酮 槲皮素 解毒酶 

分 类 号：S435.622[农业科学—农业昆虫与害虫防治]