非生物胁迫因子对大豆Sali3-2基因的调控作用  

Regulatable gene expression controlled by the promoter of Sali3-2 under different abiotic stresses

作　　者：唐玉林[1,2] 曹雁[1,2] 欧忠华[1,2] 杨悉妮[1,2] 胡恋苹[1,2] 郑易之[1,2] 

机构地区：[1]深圳大学生命科学学院,深圳518060 [2]深圳市海洋生物资源与生态环境重点实验室,深圳市微生物基因工程重点实验室,深圳518060

出　　处：《深圳大学学报（理工版）》2012年第1期73-79,共7页Journal of Shenzhen University(Science and Engineering)

基　　金：国家自然科学基金资助项目(30770184)~~

摘　　要：通过生物信息学方法对Sali3-2基因上游非编码区序列(-1 945/+1)中的顺式作用元件进行预测.发现该序列中存在铜响应元件、干旱胁迫响应元件及与低温等非生物胁迫相关元件等.在不同非生物胁迫条件下,分别对转基因烟草悬浮细胞和转基因拟南芥幼苗中Sali3-2启动子片段驱动报告基因β-葡萄糖苷酸酶(β-glucuronidase,Gus)的表达进行分析.结果表明,在转基因烟草细胞和拟南芥幼苗中,Gus基因的表达明显受Al3+胁迫的诱导和Cu2+过多的抑制;在转基因烟草细胞中,该基因的表达一定程度上受脱落酸、渗透胁迫和盐胁迫的诱导,研究结果为了解Sali3-2基因的功能及调控作用奠定了基础.Sali3-2 from soybean is a BURP gene related to stress response. To elucidate its transcriptional regula- tion characteristics, the upstream non-coding sequence （ - 1945 / ＋ 1 ） of Sali3-2 was scanned in PLACE database for searching the cis-acting regulatory elements. Several copper response elements and some c/s-element motifs related to gene induction by abseisie acid （ABA）, dehydration and cold stress were found in this sequence. The expression of reporter gene Gus driven by the promoter region of Sali3-2 was further studied in tobacco cells and Arabidopsis seedlings at different abiotic stresses. Results show that the expression of the reporter is obviously up-regulated by Al^（3＋） stress and intensely suppressed by abundance Cu^（2＋）. The expression of the reporter is slightly up-regulated by abscisic acid, osmotic stress and salt stress in transgenic tobacco cells. These findings extend our understanding of the regulation of Sali3-2 expression.

关 键 词：生物信息学 基因调控 启动子 Sali3-2基因 铜响应元件 非生物胁迫 GUS染色 

分 类 号：Q78[生物学—分子生物学] Q812