逆境诱导烟草悬浮细胞内处理小体的形成  

Stress-induced processing body formation in tobacco suspension cells

作　　者：莫蓓莘[1] 叶浩[1] 欧忠华[1] 刘丽[1] 黄彪[1] 朱双琳[1,2] 

机构地区：[1]深圳大学生命科学学院,深圳518060 [2]麦克马斯特大学健康科学系

出　　处：《深圳大学学报（理工版）》2012年第1期80-84,共5页Journal of Shenzhen University(Science and Engineering)

基　　金：国家自然科学基金资助项目(30970265);广东省自然科学基金自由申请项目基金资助(9151806001000017)~~

摘　　要：指出处理小体(processing body,P-body)在真核细胞基因表达调节过程中,特别是在逆境条件下,能为mRNA在细胞质内转运提供储存和降解场所.研究通过农杆菌转化法成功将含有处理小体荧光标记物-脱帽酶1(decapping enzyme,DCP1)与青色荧光蛋白(cyan fluorescent protein,CFP)融合蛋白基因的植物表达载体转入烟草悬浮细胞.利用激光共聚焦显微镜观察到在高温和盐胁迫下,处理小体的数量和体积都有增加.通过蔗糖密度梯度离心分析逆境条件下多核糖体的变化,发现在高温和盐胁迫下,多核糖体解体,呈现翻译抑制特征.Processing bodies（P-body） serve as key functions in regulating gene expression in eukaryotic cells, and especially under stress conditions, serve as stress-induced mRNA relocalization sites for mRNA degradation or storage. We successfully transformed P-body fluorescent marker （ DCP1 and CFP fusion protein） containing plasmid into suspension BY-2 cells. Confocal observation found that P-body increased in abundance and size under heat and salt stress conditions. Sucrose gradient ultra-centrifugation analysis demonstrated that both heat and salt stress conditions induced polysome run-off. This is characteristic of inhibition of translation initiation.

关 键 词：植物细胞学 处理小体 多核糖体 脱帽酶1 逆境胁迫 信使核糖核酸 烟草悬浮细胞-BY-2 青色荧光蛋白 

分 类 号：Q942.5[生物学—植物学] Q256