表皮葡萄球菌icaA和icaD基因表达及其与黏质物形成的关系  

Relationship between icaA,icaD gene expression and slime formation in Staphylococcus epidermidis

作　　者：费明明[1] 刘宝[1] 周树生[1] 曹晓光[1] 戴媛媛 

机构地区：[1]安徽医科大学附属省立医院ICU,合肥230001 [2]安徽医科大学附属省立医院微生物实验室,合肥230001

出　　处：《中国感染与化疗杂志》2012年第1期50-53,共4页Chinese Journal of Infection and Chemotherapy

基　　金：安徽省教育厅课题基金资助项目(KJ2009B001Z)

摘　　要：目的了解临床分离的表皮葡萄球菌所携带的icaA和icaD操纵子表达情况及其与黏质物形成之间的关系。方法收集ICU临床分离的表皮葡萄球菌62株,采用刚果红法检测其黏质物形成,PCR法检测icaA、icaD基因表达,卡方检验法进行统计学处理。结果 62株表皮葡萄球菌中产黏质物菌株有26株(41.9%);产黏质物菌株中icaA、icaD基因表达阳性的菌株分别为22株(84.6%)和19株(73.1%),icaA、icaD基因表达均为阳性有17株(65.4%),明显高于非产黏质物菌株(P<0.01);icaA、icaD基因表达均阴性为2株(7.7%),明显低于非产黏质物菌株(P<0.01)。结论 icaA、icaD基因表达对表皮葡萄球菌的黏质物产生具有重要影响,但并不是其唯一的影响因素。Objective To study the relationship between icaA, icaD gene expression and slime formation in Staphylococcus epidermidis strains isolated from ICU. Methods Slime-forming ability of 62 strains of S. epidermidis was tested on Congo red agar plates. The icaA, icaD gene expression was analyzed by PCR. The data were analyzed by Chi-square test. Results Slime production was identified in 41. 9% （26/62） of the strains. Gene icaA and icaD were expressed in 22 （84. 6%） and 19 （73.1%） of those slime-positive strains respectively. Both icaA and icaD genes were expressed in 17 （65.4%） strains. The prevalence of icaA, icaD gene expression in slime-producing S. epidermidis was higher than that in non-slime-producing S. epiderrnidis （P〈0. 01）. Two （7.7%） strains were negative for both icaA and icaD genes. Conclusions Expression of icaA and icaD genes plays an important role in slime formation in S. epidermidis.

关 键 词：表皮葡萄球菌 黏质物 icaA基因 icaD基因 

分 类 号：R378.11[医药卫生—病原生物学]