不同嫁接方法对甜瓜枯萎病抗性的影响及其生理响应  

Effects of Different Grafting Methods on Melon Resistance to Fusarium Wilt and Physiological Responses

作　　者：刘朋义[1] 别之龙[1] 

机构地区：[1]华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,湖北武汉430070

出　　处：《长江蔬菜》2011年第24期33-37,共5页Journal of Changjiang Vegetables

基　　金：国家科技支撑计划(2009BADB8B02);国家西甜瓜产业技术体系项目资助(CARS-26-16)

摘　　要：本文研究了插接、断根嫁接和靠接3种嫁接方法对甜瓜枯萎病抗性的影响。结果显示,3种嫁接方法均能显著提高甜瓜的抗枯萎病能力,但3种嫁接方法之间无显著差异。接种枯萎病菌后,嫁接苗根系活力、叶绿素含量、根系中SOD、POD、PAL活性显著高于自根苗,而MDA含量、相对电导率显著低于自根苗。以上结果表明,嫁接是提高甜瓜对枯萎病抗性的有效措施,但不同嫁接方法对甜瓜枯萎病抗性无显著影响;嫁接苗通过抗氧化系统和PAL活性的提高抵御了枯萎病菌侵染后所致的膜脂过氧化伤害,维持了根系和叶片的正常生理功能,从而提高了嫁接苗对甜瓜枯萎病的抗性。The cuttage grafting, root-cutting grafting and tongue approach grafting were used to study their effects on melon resistance to Fusarium wilt. The results showed that all the three grafting methods could significantly improve the resistance to Fusarium wilt for melon, but no differences were observed among the three grafting methods employed in this study. After incubation of Fusarium, the root vigor, chlorophyll content, activities of SOD, POD and PAL in the root increased, whereas the MDA content and relative conductivity decreased significantly in the grafted plants, as compared with the non-grafted melon plants. The enhanced resistance of grafted seedlings to Fusarium wilt could be attributed to the alleviation of oxidative stress caused by the antioxidant system and the improved activity of PAL. As a result, the physiological functions were maintained properly in the roots and leaves.

关 键 词：甜瓜 枯萎病 嫁接方法 生理变化 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]