不同抗性甜瓜接种蔓枯病菌后若干生理指标的变化  

Changes of Several Physiological Characters in Melons with Different Resistances after Inoculation with Didymella bryoniae

作　　者：王红英[1] 钱春桃[1] 张永兵 伊鸿平 郭勤卫[1] 刘佳[1] 吴明珠 陈劲枫[1] 

机构地区：[1]作物遗传与种质创新国家重点实验室.南京农业大学园艺学院,南京210095 [2]新疆维吾尔自治区农业科学院哈密瓜研究中心,乌鲁木齐830000

出　　处：《中国瓜菜》2012年第1期7-10,共4页China Cucurbits And Vegetables

基　　金：国家自然科学基金新疆联合基金项目(U1178307)

摘　　要：对不同抗性甜瓜接种蔓枯病菌后测定叶片中叶绿素、可溶性糖、可溶性蛋白含量和3种防御酶(PPO、PO、PAL)的活性。结果表明:叶绿素含量在接种后呈现先增加后减小趋势,抗病甜瓜增幅大,叶绿素含量高于感病甜瓜;可溶性糖含量在接种后先降低后升高、再降低再升高,抗病甜瓜可溶性糖含量高于感病甜瓜;可溶性蛋白含量在接种后逐渐升高,感病甜瓜峰值出现早于抗病甜瓜;3种酶活性在接种后都有不同程度提高,其中抗病甜瓜酶活性增幅高于感病甜瓜。几种酶活性变化可以用于甜瓜蔓枯病抗性鉴定。The content of chlorophyll,soluble sugar,soluble protein,and activities of three protective enzymes （PPO,PO and PAL）in leaves of the melons with different resistances were measured and analyzed after inoculation with DidymeUa bryoniae. The results showed that chlorophyll content increased at first and then decreased in both resistant and susceptible melons after inoculation. However, the level and increment of chlorophyll content were higher in resistant melon. The soluble sugar contents in both resistant and susceptible melons showed a similar pattern of reduce-increase-reduce-increase after inoculation. The soluble sugar content in the resistant melon was higher than that in the susceptible melon. The soluble protein content increased both in resistant and susceptible melons. The peak of soluble protein content occurred earlier in the susceptible melon. Activities of the three protective enzymes increased with different levels. The increase from the resistant melon was higher than that from the susceptible melon. The results obtained in this experiment could be used as indicators for gummy stem blight resistance in melon breeding.

关 键 词：甜瓜 蔓枯病菌 叶绿素 可溶性糖 可溶性蛋白 PPO PO PAL 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]