豇豆根腐病病原分离鉴定及其核糖体rDNA-ITS序列分析  

Pathogeny Isolation and Identification of Cowpea Root Rot Disease and Sequencing of Ribosomal rDNA-ITS

作　　者：吴仁锋[1] 杨绍丽[1] 万鹏[2] 黄薇[2] 

机构地区：[1]武汉市蔬菜科学研究所,武汉430065 [2]湖北省农业科学院植保土肥研究所,武汉430064

出　　处：《湖北农业科学》2011年第24期5107-5110,共4页Hubei Agricultural Sciences

基　　金：武汉市科技攻关计划[2009]19号

摘　　要：采用形态学及分子生物学的方法对采集自武汉的豇豆根腐病分离物进行形态学和分子生物学鉴定。结果表明,菌株JGF形态学上为茄腐皮镰孢菌;对JGF菌株的核糖体RNA基因内转录间隔区(rD-NA-ITS)进行了PCR扩增和序列测定,并在GenBank中进行了Blast搜索和比对分析,根据rDNA-ITS序列分析结果结合豇豆病株症状和病菌的形态学特征,认为武汉地区豇豆根腐病的病原菌为茄腐皮镰孢菌(Fusarium solani Schl.)The fungal isolate JGF from cowpea root rot disease,which were collected from Wuhan,were studied morphologically and molecularly.The results showed that the isolate JGF were morphological Fusarium solani Schl.The rDNA-ITS genes of isolate JGF were amplified and sequenced.Based on the Blast search and the alignment analysis in GenBank database combined with morphological characters,the pathogen of cowpea root rot disease was identified as Fusarium solani Schl.

关 键 词：豇豆根腐病 形态学 分子鉴定 rDNA-ITS分析 FUSARIUM SOLANI Schl. 

分 类 号：S436.43[农业科学—农业昆虫与害虫防治]